Measuring the Effect of PDGF on Fibroblasts Using Glioma Cell Lines

  • Nobusada Shinoura
  • Tatsuya Kondou
  • Masumi Yoshioka


The platelet-derived growth factor (PDGF) was first identified as having a potent growth-promoting activity in bovin serum, followed by evidence of this activity over glia, smooth muscle cell, and fibroblasts. Purification of PDGF revealed a heterodimeric glycoprotein composed of two basic polypeptide chains, A and B, in a disulfide linkage. Many PDGF-like growth factors, called cellular PDGF (PDGFc), were found without platelets, and were composed of AA, AB, and BB. We investigated PDGFc using 4 cell lines and, in this paper, we describe the existence of PDGF (AA, BB) in tumor cell lines and the effects of PDGF (AA, BB) on the fibroblasts and on the secretion of PDGF from the tumor cell lines.


Conditioned Medium Tumor Cell Line Thymidine Incorporation Glioma Cell Line Mitogenic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Balk SD (1971) Calcium as a regulator of the proliferation of normal, but not of transformed, chicken fibroblasts in a plasma-containing medium Proc Natl Acad Sci USA 68: 271–275PubMedCrossRefGoogle Scholar
  2. 2.
    Kohler N, Lipton A (1971) Platelets as a source of fibroblast growth-promoting activity Exp Cell Res 98: 297–301Google Scholar
  3. 3.
    Ross R, Glomset J, Kariya B, Harker L (1974) A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci USA 71: 1207–1210PubMedCrossRefGoogle Scholar
  4. 4.
    Westermark B, Westeson A (1976) A platelet factor stimulating human normal glial cells. Exp Cell Res 98: 170–174PubMedCrossRefGoogle Scholar
  5. 5.
    Antoniades, HN, Stathkos D, Scher CD (1975) Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells. Proc Natl Acad Sci USA 72: 2635–2639PubMedCrossRefGoogle Scholar
  6. 6.
    Johnsson A, Heldin CH, Westermark B, Wasteson A (1982) Platelet-derived growth factor Identification of constituent polypeptide chains. Biochem Biophys Res Commun 104: 66–74PubMedCrossRefGoogle Scholar
  7. 7.
    Shimokado K, Raines EW, Medtes DK, Barrett TB, Benditt EP, Ross R (1985) A significant part of macrophage-derived growth factor consists of at least two forms of PDGF. Cell 43: 277–286PubMedCrossRefGoogle Scholar
  8. 8.
    Seifert RA, Schwartz SM, Bowen-Pope DF (1984) Developmentally regulated production of platelet-derived growth factor-like molecules. Nature 311: 669–671PubMedCrossRefGoogle Scholar
  9. 9.
    Goustin AS, Betsholts C, Pfeifer-Ohlsson S, Persson H, Rydnert J, Bywater M, Holmgren G, Heldin CH, Westermark B, Ohosson R (1985) Co-expression of the sis and myc proto-oncogenes in developing human placenta suggests autocrine control of trophoblast growth. Cell 41: 301–312PubMedCrossRefGoogle Scholar
  10. 10.
    Heldin CH, Johnsson A, Wennergren S, Warnstedt C, Betsholtz C, Westermark C (1986) A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF-A chains. Nature 319: 511–514PubMedCrossRefGoogle Scholar
  11. 11.
    Nister M, Hammacher A, Meilstrom K, Siegbahn A, Ronnstrand L, Westermark K, Heldin CH (1988) A glioma-derived PDGF-A chain homodimer has differnet functional activities from a PDGF-AB heterodimer purified from human platelets. Cell 52: 791–799PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Nobusada Shinoura
  • Tatsuya Kondou
  • Masumi Yoshioka
    • 1
  1. 1.National Medical CenterShinjuku-ku, Tokyo, 162Japan

Personalised recommendations