Measuring the Effect of PDGF on Fibroblasts Using Glioma Cell Lines

  • Nobusada Shinoura
  • Tatsuya Kondou
  • Masumi Yoshioka


The platelet-derived growth factor (PDGF) was first identified as having a potent growth-promoting activity in bovin serum, followed by evidence of this activity over glia, smooth muscle cell, and fibroblasts. Purification of PDGF revealed a heterodimeric glycoprotein composed of two basic polypeptide chains, A and B, in a disulfide linkage. Many PDGF-like growth factors, called cellular PDGF (PDGFc), were found without platelets, and were composed of AA, AB, and BB. We investigated PDGFc using 4 cell lines and, in this paper, we describe the existence of PDGF (AA, BB) in tumor cell lines and the effects of PDGF (AA, BB) on the fibroblasts and on the secretion of PDGF from the tumor cell lines.


Conditioned Medium Tumor Cell Line Thymidine Incorporation Glioma Cell Line Mitogenic Activity 


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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Nobusada Shinoura
  • Tatsuya Kondou
  • Masumi Yoshioka
    • 1
  1. 1.National Medical CenterShinjuku-ku, Tokyo, 162Japan

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