PDGF-Related Proteins in Human Brain Tumors

  • Takaharu Nakamura
  • Iwao Takeshita
  • Masashi Fukui


The platelet-derived growth factor (PDGF) is one of the well known mitogenic factors for glial cells and connective tissue cells [1–3]. It is suggested that PDGF-related protein has an autocrinic effect and participates in malignant neoplastic transformation [4–6]. The mRNA expression of A and B chains was detected in some glioma cells [7]. It was reported that meningiomas expressed both PDGF and its receptor [8]. Expression of the PDGF-related proteins in the various types of brain tumors has not yet been estimated. We have detected 17 kilodalton (kd) PDGF-related proteins in glioma cells [9], and obtained rabbit antibody against glioma-derived PDGF-related protein, with which we studied the presence of PDGF-related proteins in various cultured human cells and brain tumors.


Brain Tumor Glioma Cell U251MG Cell Nonreduced Condition Human Brain Tumor 
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  1. 1.
    Heldin C-H, Westermark B, Wasteson A (1979) Platelet-derived growth factor: Purification and partial characterization. Proc Natl Acad Sci USA 76: 3722–3726PubMedCrossRefGoogle Scholar
  2. 2.
    Ross R, Raines EW, Bowen-Pope DF (1986) The biology of platelet-derived growth factor. Cell 46: 155–169PubMedCrossRefGoogle Scholar
  3. 3.
    Westermark B, Wasteson Å (1976) A platelet factor stimulating human normal glial cells. Exp Cell Res 78: 170–174CrossRefGoogle Scholar
  4. 4.
    Hermansson M, Nister M, Betsholtz C, Heldin C-H, Westermark B, Funa K (1988) Endothelial cell hyperplasia in human glioblastoma: Coexpression of mRNA for platelet-derived growth factor ( PDGF) B chain and PDGF receptor suggests autocrine growth stimulation. Proc Natl Acad Sci USA 85: 7748–7752PubMedCrossRefGoogle Scholar
  5. 5.
    Heldin C-H, Betsholtz C, Johnsson A, Westermark B (1986) Role of PDGF-like growth factors in malignant transformation. Cancer Rev. 2: 34–47Google Scholar
  6. 6.
    Sporn MB, Roberts AB (1985) Autocrine growth factors and cancer. Nature 313: 745–747PubMedCrossRefGoogle Scholar
  7. 7.
    Betsholtz C, Johnsson A, Heldin C-H, Westermark B, Lind P, Urdea MS, Eddy R, Shows TB, Philpot K, Mellor AL, Knott TJ, Scott J (1986) cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature 320: 695–699Google Scholar
  8. 8.
    Maxwell M, Galanopoulos T, Hedley-Whyte ET, Black PM, Antoniades HN (1990) Human meningiomas co-express platelet-derived growth factor ( PDGF) and PDGF- receptor genes and their protein products. Int J Cancer 46: 16–21PubMedCrossRefGoogle Scholar
  9. 9.
    Nakamura T, Takeshita I, Fukui M (1989) PDGF-related proteins in human gliomas. Neuroimmunolog Res 2: 150–154Google Scholar
  10. 10.
    Ponten J, Westermark B (1978) Properties of human malignant glioma cells in vitro. Med Biol 56: 184–193PubMedGoogle Scholar
  11. 11.
    Yamazaki K (1982) Tumorigenicity of established human glioma cell lines in lasat and nude mice—in relation to differentiation and anaplasia of glioma cells—(in Japanese with an English summary). Neuropathol. 3: 29–38Google Scholar
  12. 12.
    Beckerle MC (1986) Identification of a new protein localized at sites of cell substrate adhesion. J Cell Biol 103: 1679–1687PubMedCrossRefGoogle Scholar
  13. 13.
    Shiraishi T, Morimoto S, Itoh K, Sato H, Onishi T, Ogihara T (1989) Radioimmunoassay of human platelet-derived growth factor using monoclonal antibody toward a synthetic 73–97 fragment of its B-chain. Clin Chim Acta 184: 65–74PubMedCrossRefGoogle Scholar
  14. 14.
    Fukui M, Iwaki T, Sawa H, Inoue T, Takeshita I, Kitamura K (1986) Proliferative activity of meningiomas as evaluated by bromodeoxyuridine uptake examination. Acta Neurochir (Wien) 81: 135–141CrossRefGoogle Scholar
  15. 15.
    Huang JS, Huang SS, Deuel TF (1984) Transforming protein of simian sarcoma virus stimulates autocrine growth of ssv-transformed cells through PDGF cell- surface receptors. Cell 39: 79–87PubMedCrossRefGoogle Scholar
  16. 16.
    Keating MT, Williams LT (1988) Autocrine stimulation of intracellular PDGF receptors in v-sis-transformed cells. Science 239: 914–916PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Takaharu Nakamura
  • Iwao Takeshita
  • Masashi Fukui
    • 1
  1. 1.Department of Neurosurgery, Neurological Institute, Faculty of MedicineKyushu UniversityFukuoka, 812Japan

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