Quantitative Autoradiographical Evaluation of Protein Synthesis in a Brain Tumor Model Using 14C-labeled Valine
Since tumor tissue grows rapidly, it can be assumed that a large amount of protein has to be synthesized in the tissue. Previous studies have already shown evidence that a very high exogenous uptake of amino acid is present in human brain tumor [1,2]. The studies were done by positron emission tomography (PET) using 11C-DL-valine, 11C-DL-tryptophan , and L-11C-methionine . An autoradiographical method for the measurement of the rate of protein synthesis has also been introduced [3,4]. A variation of this method has been applied to some experimental brain tumors as well . The kinetic model for L-1-14C- leucine incorporation into proteins, described by Smith et al. , was applied to L-1-14C-valine, and the rate constants were measured in several normal brain structures . In this paper, we describe the measurement of the rate constants and the rate of valine incorporation into proteins in AA ascites tumors implanted into rat brains. The reliability and the applicability of the kinetic model to the tumor model is also discussed.
KeywordsPositron Emission Tomography Acid Soluble Fraction Precursor Pool Brain Tumor Model Local Cerebral Glucose Utilization
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