The Proliferative Potential After Hyperthermia and Irradiation on a Human Glioma Cell Line
The lethal effects of hyperthermia and irradiation on glioma cells have been demonstrated both in vitro and in vivo. In general, cells in the S-phase are the most resistant to X-rays, whereas they are the most sensitive to hyperthermia. In this report, the effect of hyperthermia or irradiation on cultured glioma cells was examined by colony formation assay. The effect of these treatments on the proliferative potential of the glioma cells was investigated with an immunohistochemical technique using monoclonalantibody (MoAb) against bromodeoxyuridine (BrdU) and MoAb Ki-67.
KeywordsGlioma Cell Malignant Glioma Colony Formation Assay Human Glioma Cell Tissue Culture Dish
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