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The Proliferative Potential After Hyperthermia and Irradiation on a Human Glioma Cell Line

  • Hideaki Shigematsu
  • Kazuyuki Tsuno
  • Takashi Matsuhisa
  • Tomohide Maeshiro
  • Nobuya Mishima
  • Kengo Matsumoto
  • Tomohisa Furuta
  • Minoru Arimori
  • Akira Nishimoto

Abstract

The lethal effects of hyperthermia and irradiation on glioma cells have been demonstrated both in vitro and in vivo. In general, cells in the S-phase are the most resistant to X-rays, whereas they are the most sensitive to hyperthermia. In this report, the effect of hyperthermia or irradiation on cultured glioma cells was examined by colony formation assay. The effect of these treatments on the proliferative potential of the glioma cells was investigated with an immunohistochemical technique using monoclonalantibody (MoAb) against bromodeoxyuridine (BrdU) and MoAb Ki-67.

Keywords

Glioma Cell Malignant Glioma Colony Formation Assay Human Glioma Cell Tissue Culture Dish 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Hideaki Shigematsu
  • Kazuyuki Tsuno
  • Takashi Matsuhisa
  • Tomohide Maeshiro
  • Nobuya Mishima
  • Kengo Matsumoto
  • Tomohisa Furuta
  • Minoru Arimori
  • Akira Nishimoto
    • 1
  1. 1.Department of Neurological SurgeryOkayama University Medical SchoolOkayama, 700Japan

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