Evaluation of the Proliferation on Brain Tumor Cells and the Endothelial Cells of Tumor Vessels Using Immunohistochemical Study with Anti-BrdU Monoclonal Antibody and Factor VIII-Related Antigen

  • Mitsusuke Miyagami
  • Eishi Kasahara
  • Takashi Tsubokawa


Malignant brain tumors, especially those of the astrocytic series, are characterized by neovascularity and endothelial proliferation. To understand the biology of these tumors, it is important to study the properties of the abnormal intratumoral endothelial cells. Cytokinetic studies of human brain tumors, using bromodeoxyuridine (BrdU), provide a far more reliable method to determine the proliferative rate of cells than the measurement of angiogenesis and counts of mitotic figures by light microscopy [1]. Tissue culture studies have confirmed the synthesis of factor VHI-related antigen (FVIIIR:Ag) in endothelial cells [2,3]. The immunohistochemical demonstration of FVIIIR:Ag has recently been used as a marker for endothelial cells in neoplastic diseases [4–6].


Brain Tumor Pituitary Adenoma Glioblastoma Multiforme Malignant Brain Tumor Blood Vessel Wall 
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Copyright information

© Springer-Verlag Tokyo 1991

Authors and Affiliations

  • Mitsusuke Miyagami
  • Eishi Kasahara
  • Takashi Tsubokawa
    • 1
  1. 1.Department of Neurological Surgery, School of MedicineNihon UniversityChiyodaku, 101Japan

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