A Combined Method for Immunohistochemistry and Autoradiographic Measurement of Focal Cerebral Blood Flow in Investigation of Pathophysiological Roles of Microcirculation in Cerebral Ischemia
Measurement of focal cerebral blood flow (CBF) using quantitative autoradiography has provided valuable information in the investigation of cerebral ischemia . However, the measurement of CBF alone has not been very successful for elucidation of the pathophysiological mechanism of cerebral ischemia and the selective tissue vulnerability, partly because of the lack of a sensitive morphological method for visualization of early ischemic damage. Since immunohistochemical techniques can detect early ischemic damage [2, 3], we aimed, in the present study, to establish a combined auto radiographic and immunohistochemical method for exact measurement of focal CBF in the area of an early immunohistochemical ischemic lesion. A more detailed description of the present work appears elsewhere .
KeywordsCerebral Blood Flow Cerebral Ischemia Ischemic Lesion Mongolian Gerbil Immunohistochemical Reaction
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