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A Combined Method for Immunohistochemistry and Autoradiographic Measurement of Focal Cerebral Blood Flow in Investigation of Pathophysiological Roles of Microcirculation in Cerebral Ischemia

  • Masayasu Matsumoto
  • Takao Hatakeyama
  • Shotaro Yoneda
  • Kazufumi Kimura
  • Takenobu Kamada
  • Takehiko Yanagihara

Abstract

Measurement of focal cerebral blood flow (CBF) using quantitative autoradiography has provided valuable information in the investigation of cerebral ischemia [1]. However, the measurement of CBF alone has not been very successful for elucidation of the pathophysiological mechanism of cerebral ischemia and the selective tissue vulnerability, partly because of the lack of a sensitive morphological method for visualization of early ischemic damage. Since immunohistochemical techniques can detect early ischemic damage [2, 3], we aimed, in the present study, to establish a combined auto radiographic and immunohistochemical method for exact measurement of focal CBF in the area of an early immunohistochemical ischemic lesion. A more detailed description of the present work appears elsewhere [4].

Keywords

Cerebral Blood Flow Cerebral Ischemia Ischemic Lesion Mongolian Gerbil Immunohistochemical Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Mies G, Kloiber O, Drewes LR, Hossmann KA (1984) Cerebral blood flow and regional potassium distribution during focal ischemia of gerbil brain. Ann Neural 16: 232–237CrossRefGoogle Scholar
  2. 2.
    Yanagihara T, Yoshimine T, Morimoto K, Yamamoto K, Homburger HA (1985) Immunohistochemical investigation of cerebral ischemia in gerbils. J Neuropath Exp Neurol 44: 204–215PubMedCrossRefGoogle Scholar
  3. 3.
    Matsumoto M, Yamamoto K, Homburger HA, Yanagihara T (1987) Early detection of cerebral ischemic damage and repair process in the gerbil by use of an immunohistochemical technique. Mayo C1in Proc 62: 460–472Google Scholar
  4. 4.
    Matsumoto M, Hatakeyama T, Yamamoto K, Yanagihara T (1987) A combined method for measurement of cerebral blood flow and immunohistochemistry for investigation of cerebral ischemia. Brain Res 424: 231–238PubMedCrossRefGoogle Scholar
  5. 5.
    Matsumoto M, Kimura K, Fujisawa A, Matsuyama T, Asai T, Uyama O, Yoneda S, Abe H (1982) Regional blood flows measured in Mongolian gerbil by a modified microsphere method. Am J Physiol 242: H990-H995PubMedGoogle Scholar
  6. 6.
    Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with iodo[14C]antipyrine. Am J Physiol 234: H59-H66PubMedGoogle Scholar
  7. 7.
    Loskota WJ, Lomax P, Verty MA (1974) A stereotaxic atlas of the Mongolian gerbil brain (Meriones unguiculatus). Ann Arbor Science Publishers, Ann ArborGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1988

Authors and Affiliations

  • Masayasu Matsumoto
    • 1
    • 2
  • Takao Hatakeyama
    • 1
  • Shotaro Yoneda
    • 2
  • Kazufumi Kimura
    • 2
  • Takenobu Kamada
    • 2
  • Takehiko Yanagihara
    • 1
  1. 1.Department of NeurologyMayo ClinicRochesterUSA
  2. 2.First Department of Internal MedicineOsaka University Medical SchoolFukushima-ku, Osaka, 553Japan

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