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A Combined Method for Immunohistochemistry and Autoradiographic Measurement of Focal Cerebral Blood Flow in Investigation of Pathophysiological Roles of Microcirculation in Cerebral Ischemia

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Microcirculation in Circulatory Disorders

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Abstract

Measurement of focal cerebral blood flow (CBF) using quantitative autoradiography has provided valuable information in the investigation of cerebral ischemia [1]. However, the measurement of CBF alone has not been very successful for elucidation of the pathophysiological mechanism of cerebral ischemia and the selective tissue vulnerability, partly because of the lack of a sensitive morphological method for visualization of early ischemic damage. Since immunohistochemical techniques can detect early ischemic damage [2, 3], we aimed, in the present study, to establish a combined auto radiographic and immunohistochemical method for exact measurement of focal CBF in the area of an early immunohistochemical ischemic lesion. A more detailed description of the present work appears elsewhere [4].

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References

  1. Mies G, Kloiber O, Drewes LR, Hossmann KA (1984) Cerebral blood flow and regional potassium distribution during focal ischemia of gerbil brain. Ann Neural 16: 232–237

    Article  CAS  Google Scholar 

  2. Yanagihara T, Yoshimine T, Morimoto K, Yamamoto K, Homburger HA (1985) Immunohistochemical investigation of cerebral ischemia in gerbils. J Neuropath Exp Neurol 44: 204–215

    Article  PubMed  CAS  Google Scholar 

  3. Matsumoto M, Yamamoto K, Homburger HA, Yanagihara T (1987) Early detection of cerebral ischemic damage and repair process in the gerbil by use of an immunohistochemical technique. Mayo C1in Proc 62: 460–472

    CAS  Google Scholar 

  4. Matsumoto M, Hatakeyama T, Yamamoto K, Yanagihara T (1987) A combined method for measurement of cerebral blood flow and immunohistochemistry for investigation of cerebral ischemia. Brain Res 424: 231–238

    Article  PubMed  CAS  Google Scholar 

  5. Matsumoto M, Kimura K, Fujisawa A, Matsuyama T, Asai T, Uyama O, Yoneda S, Abe H (1982) Regional blood flows measured in Mongolian gerbil by a modified microsphere method. Am J Physiol 242: H990-H995

    PubMed  CAS  Google Scholar 

  6. Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with iodo[14C]antipyrine. Am J Physiol 234: H59-H66

    PubMed  CAS  Google Scholar 

  7. Loskota WJ, Lomax P, Verty MA (1974) A stereotaxic atlas of the Mongolian gerbil brain (Meriones unguiculatus). Ann Arbor Science Publishers, Ann Arbor

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Neurology, Mayo Clinic, Rochester, MN, 55905, USA

    Masayasu Matsumoto, Takao Hatakeyama & Takehiko Yanagihara

  2. First Department of Internal Medicine, Osaka University Medical School, 1-50, Fukushima 1-chome, Fukushima-ku, Osaka, 553, Japan

    Masayasu Matsumoto, Shotaro Yoneda, Kazufumi Kimura & Takenobu Kamada

Authors
  1. Masayasu Matsumoto
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  2. Takao Hatakeyama
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  3. Shotaro Yoneda
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  4. Kazufumi Kimura
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  5. Takenobu Kamada
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  6. Takehiko Yanagihara
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Editor information

Editors and Affiliations

  1. National Cardiovascular Center of Japan, Suita, Osaka, 565, Japan

    Hisao Manabe M.D. (President) (President)

  2. University of California, San Diego, La Jolla, CA, 92093, USA

    Benjamin W. Zweifach Ph.D. (Professor Emeritus of Bioengineering) (Professor Emeritus of Bioengineering)

  3. University of Heidelberg, D-6900, Heidelberg, Federal Republic of Germany

    Konrad Messmer M.D. (Professor of Experimental Surgery) (Professor of Experimental Surgery)

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© 1988 Springer-Verlag Tokyo

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Matsumoto, M., Hatakeyama, T., Yoneda, S., Kimura, K., Kamada, T., Yanagihara, T. (1988). A Combined Method for Immunohistochemistry and Autoradiographic Measurement of Focal Cerebral Blood Flow in Investigation of Pathophysiological Roles of Microcirculation in Cerebral Ischemia. In: Manabe, H., Zweifach, B.W., Messmer, K. (eds) Microcirculation in Circulatory Disorders. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68078-9_70

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  • DOI: https://doi.org/10.1007/978-4-431-68078-9_70

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68080-2

  • Online ISBN: 978-4-431-68078-9

  • eBook Packages: Springer Book Archive

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