Advertisement

Angiotensin Converting Enzyme Activity in Brain Microvessels of Rats

  • Satoko Itoh
  • Yoshiharu Kanayama
  • Hiromi Inariba
  • Mikio Okamura
  • Kazuo Takaori
  • Takatoshi Inoue
  • Tadanao Takeda

Abstract

Angiotensin converting enzyme (ACE) is a carboxyl terminal dipeptidyl dipeptidase, which is known to convert angiotensin I to angiotensin II (Ang II) and to hydrolyze bradykinin and enkephalin. In the brain, ACE is located in endothelial cells of capillaries throughout the brain, in the subfornical organ, and in the brush border of choroidal epithelial cells in contact with cerebrospinal fluid [1]. Administration of Ang II into the brain ventricles induces thirst [2], a rise in arterial blood pressure, and secretion of vasopressin [3]. Recently, the specific binding of Ang II to the membrane fraction of canine brain microvessels was detected, suggesting the role of Ang II in regulation of the brain microcirculation and transport across the blood-brain barrier [4]. Since ACE in the brain microvessels might affect the brain microcirculation by modulating the local production of Ang II, we measured ACE activity in brain microvessels obtained from 13-week-old spontaneously hypertensive rats (SHR), Wistar-Kyoto rats, and SHR of the same age under antihypertensive therapy to clarify the effect of hypertension on the brain microvessels.

Keywords

Angiotensin Converting Enzyme Angiotensin Converting Enzyme Activity Brain Microvessels Histidine Decarboxylase Subfornical Organ 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Brownfield MS, Reid IA, Ganten D, Ganong WF (1982) Differential distribution of immunoreactive angiotensin and angiotensin-converting enzyme in rat brain. Neuroscience 7: 1759–1769PubMedCrossRefGoogle Scholar
  2. 2.
    Epstein AN, Fitzsimons JT, Rolls BJ (1969) Drinking induced by injection of angiotensin into the brain of the rat. J Physiol (London) 210: 457–474Google Scholar
  3. 3.
    Severs WB, Daniels-Severs AE (1973) Effects of angiotensin on the central nervous system. Pharmacol Rev 25: 415–440PubMedGoogle Scholar
  4. 4.
    Speth RC, Haric SI (1985) Angiotensin II receptor binding sites in brain microvessels. Proc Natl Acad Sci USA 82: 6340–6343PubMedCrossRefGoogle Scholar
  5. 5.
    Goldstein GW, Wolinsky JS, Csejtey J, Diamond I (1975) Isolation of metabolically active capillaries from rat brain. J Neurochem 25: 715–717PubMedCrossRefGoogle Scholar
  6. 6.
    Horiuchi M, Fujimura K, Terashima T, Iso T (1982) Method for determination of angiotensin-converting enzyme activity in blood and tissue by high-performance liquid chromatography. J Chromatog 233: 123–130CrossRefGoogle Scholar
  7. 7.
    Orlowsky M, Meister A (1965) Isolation of γ-glutamyltranspeptidase from hog kidney. J Biol Chem 240: 338–347Google Scholar
  8. 8.
    Brecher P, Tercyac A, Gavras H, Chobanian A V (1978) Peptidyl dipeptidase in rabbit brain microvessels. Biochim Biophys Acta 526: 537–546PubMedGoogle Scholar
  9. 9.
    Del Vecchio PJ, Smith JR (1982) Aging of endothelium in culture: Decrease in angiotensin-converting enzyme activity. Cell Biol Int Reports 6: 379–384CrossRefGoogle Scholar
  10. 10.
    Rosen LA, Hollis TM, Sharma MG (1974) Alterations in bovine endothelial histidine decarboxylase activity following exposure to shearing stresses. Exp Mol Pathol 20: 329–343PubMedCrossRefGoogle Scholar
  11. 11.
    Shepro D, D’Amore PA (1984) Handbook of physiology. The cardiovascular system: IV. American Physiological Society, Bethesda, Maryland, pp 103–164Google Scholar

Copyright information

© Springer-Verlag Tokyo 1988

Authors and Affiliations

  • Satoko Itoh
  • Yoshiharu Kanayama
  • Hiromi Inariba
  • Mikio Okamura
  • Kazuo Takaori
  • Takatoshi Inoue
  • Tadanao Takeda
    • 1
  1. 1.First Department of Internal MedicineOsaka City University Medical SchoolAbeno-ku, Osaka, 545Japan

Personalised recommendations