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Microcirculatory and Ionic Responses to Ischemia in the Mongolian Gerbil

  • Avraham Mayevsky

Abstract

The Mongolian gerbil (Meriones unguiculatus) is a very useful animal model for studying brain responses to partial or complete ischemia [1–5]. Various aspects of brain functions and responses have been studied in this model exposed to ischemic conditions. When cerebral blood flow diminished due to the ischemia [6], inhibition of the Na+K+ ATPase occurred and, as a result, extracellular K+ level increased [7, 8]. In 1978, we described the effects of ischemia on the metabolic activity in the gerbil brain using the surface fluorometry technique to monitor reduced nicotinamide adenine dinucleotide (NADH) redox state [9]. In ischemia, we described a new phenomenon defined as secondary reflectance increase (SRI), [10]; this event is associated with ionic homeostasis disturbances which occur under energy deprivation conditions. To study this microcirculatory phenomenon under ischemia, the multiparametric monitoring approach [8] was used. Metabolic activity was measured by monitoring tissue surface O2 tension by a surface electrode. Intramitochondrial NADH redox state was evaluated by the surface fluorometry reflectometry technique described in detail previously [11]. The reflected light at the excitation wavelength (336 nm) measured simultaneously with the fluorescence light (450 nm) was used to correct the fluorescence signal for hemodynamic artifacts. This signal is largely dependent upon blood volume at the measurement site. Extracellular K+ activity was measured by a surface valinomycine electrode located in the multiprobe assembly.

Keywords

Mongolian Gerbil Carotid Artery Occlusion Meriones Unguiculatus Ionic Response Gerbil Brain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Levine S, Payan H (1966) Effects of ischemia and other procedures on the brain and retina of the gerbil. Exp Neurol 16: 255–262PubMedCrossRefGoogle Scholar
  2. 2.
    Kahn K (1972) The natural course of experimental cerebral infarction in the gerbil. Neurology 22: 510–515PubMedGoogle Scholar
  3. 3.
    Berry K, Wisniewski HM, Svarzbein L, Baez S (1975) On the relationship of brain vasculature to production of neurological deficit and morphological changes following acute unilateral common carotid artery ligation in gerbils. J Neurol Sci 25: 75–92PubMedCrossRefGoogle Scholar
  4. 4.
    Molinari GF, Laurent JP (1976) A classification of experimental models of brain ischemia. Stroke 7: 14–17CrossRefGoogle Scholar
  5. 5.
    Robinson DG (1980) The gerbil stroke model. Gerbil Digest 7 (2): 1–4Google Scholar
  6. 6.
    Kelly RA, Halsey JH (1976) Comparison of local blood flow and oxygen availability at the same locus in the ischemic gerbil brain. Stroke 7: 274–278.CrossRefGoogle Scholar
  7. 7.
    Mayevsky A, Crowe W, Mela L (1980) The interrelation between brain oxidative metabolism and extracellular potassium in the unanesthetized gerbil. Neurol Res 1: 213–226PubMedGoogle Scholar
  8. 8.
    Friedli CM, Sclarsky DL, Mayevsky A (1982) A new multi probe assembly for surface monitoring of ionic metabolic and electrical activities in the awake brain. Am J Physiol 243: R462–R469PubMedGoogle Scholar
  9. 9.
    Mayevsky A, Bar-Sagie D, Levy N, Amosi, M (1978) The effects of ischemia on the metabolic response of the cerebral cortex of the gerbil. Fed Proc 37: 497 (Abstr 1495)Google Scholar
  10. 10.
    Zarchin N, Mayevsky A (1981) The effects of age on the metabolic and electrical responses to decapitation in the awake anesthetized rat brain. Mechan Aging Devel 16: 285–294CrossRefGoogle Scholar
  11. 11.
    Mayevsky A (1984) Brain NADH redox state monitored in vivo by fiber optic surface fluorometry. Brain Res Rev 7: 49–68CrossRefGoogle Scholar
  12. 12.
    Mayevsky A (1983) Multiparameter monitoring of the awake brain under hyperbaric oxygenation. J Appl Physiol 54: 740–748PubMedGoogle Scholar
  13. 13.
    Levy DE, Brierley JB (1974) Communications between vertebrobasilar and carotid arterial circulations in the gerbil. Exp Neurol 45: 503–508PubMedCrossRefGoogle Scholar
  14. 14.
    Ginsberg MD, Mela L, Wrobel-Kuhl K, Reivich M (1977) Mitochondrial metabolism following bilateral cerebral iscehmia in the gerbil. Ann Neurol 1: 519–527PubMedCrossRefGoogle Scholar
  15. 15.
    Mayevsky A (1978) Pyridine nucleotide oxidation-reduction state of the cerebral cortex in the awake gerbil. J Neurosci Res 3: 369–374PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Tokyo 1988

Authors and Affiliations

  • Avraham Mayevsky
    • 1
  1. 1.Department of Life SciencesBar-Ilan UniversityRamat-GanIsrael

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