A Computerized System for Spot Detection and Analysis of Two-Dimensional Electrophoresis Images
The 2-dimensional electrophoresis technique is very powerful to separate complex mixtures of proteins. It is estimated that it is possible to separate 19,000 proteins on a single gel (O’Farrel 1975; Anderson 19 77). Because of this large number, the only practical solution for using 2-dimensional electrophoresis is through a computerized system. The main tasks of the system are:
noise reduction or elimination,
spot detection or separation,
normalization and standardization.
KeywordsComputerize System Array Processor High Frequency Noise Protein Density Spot Detection
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- Anderson ML et NG (1977) High resolution two-dimensional separation of human plasma proteins. Proc. Natl. Acad. Sci. USA, 5421–5425.Google Scholar
- Coultec BP, Anderson NG (1981) The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns. In Clin. Chem., vol. 27, 11, p. 1807–1820.Google Scholar
- Miller MJ et al. (1982) Computer analysis of two-dimensional gels: semi-automatic matching. Clin. Chem., vol. 28, 4, p. 867.Google Scholar
- O’Farrel PH (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.Google Scholar
- Wiederkehr F, Vonderschmitt DJ (submitted 1985) Zweidimensionale Elektrophorese des liquor cerebrosbinalis verschiedener neurologischer Patienten.Google Scholar
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