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A Computerized System for Spot Detection and Analysis of Two-Dimensional Electrophoresis Images

  • R. Appel
  • M. Funk
  • C. Pellegrini
  • D. Hochstrasser
  • A. F. Müller

Abstract

The 2-dimensional electrophoresis technique is very powerful to separate complex mixtures of proteins. It is estimated that it is possible to separate 19,000 proteins on a single gel (O’Farrel 1975; Anderson 19 77). Because of this large number, the only practical solution for using 2-dimensional electrophoresis is through a computerized system. The main tasks of the system are:
  • image digitization,

  • noise reduction or elimination,

  • spot detection or separation,

  • normalization and standardization.

Keywords

Computerize System Array Processor High Frequency Noise Protein Density Spot Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson ML et NG (1977) High resolution two-dimensional separation of human plasma proteins. Proc. Natl. Acad. Sci. USA, 5421–5425.Google Scholar
  2. Coultec BP, Anderson NG (1981) The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns. In Clin. Chem., vol. 27, 11, p. 1807–1820.Google Scholar
  3. Harrington MG, Merril CR, Goldman G, Xu XH, McFartin DE (1984) Two-dimensional electrophoresis of cerebrospinal fluid proteins in multiple sclerosis and various neurological deseases. In electrophoresis, vol. 5, p. 236–245.CrossRefGoogle Scholar
  4. Miller MJ et al. (1982) Computer analysis of two-dimensional gels: semi-automatic matching. Clin. Chem., vol. 28, 4, p. 867.Google Scholar
  5. O’Farrel PH (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.Google Scholar
  6. Wiederkehr F, Vonderschmitt DJ (submitted 1985) Zweidimensionale Elektrophorese des liquor cerebrosbinalis verschiedener neurologischer Patienten.Google Scholar

Copyright information

© Springer-Verlag Tokyo 1985

Authors and Affiliations

  • R. Appel
    • 1
  • M. Funk
    • 1
  • C. Pellegrini
    • 1
  • D. Hochstrasser
    • 2
  • A. F. Müller
    • 2
  1. 1.Centre Universitaire d’informatiqueGeneva 4Switzerland
  2. 2.Hôpital Cantonal UniversitaireGeneva 4Switzerland

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