A Computerized System for Spot Detection and Analysis of Two-Dimensional Electrophoresis Images
The 2-dimensional electrophoresis technique is very powerful to separate complex mixtures of proteins. It is estimated that it is possible to separate 19,000 proteins on a single gel (O’Farrel 1975; Anderson 19 77). Because of this large number, the only practical solution for using 2-dimensional electrophoresis is through a computerized system. The main tasks of the system are:
noise reduction or elimination,
spot detection or separation,
normalization and standardization.
KeywordsComputerize System Array Processor High Frequency Noise Protein Density Spot Detection
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