Systematic Detection of DNA Alteration in Cancer Tissue
Tumorigenesis is a multistep process involving both epigenetic and genetic alterations [1,2]. The identification of gain of function mutations in proto-oncogenes and loss of function mutations in tumor suppressor genes has provided a rationale for understanding tumorigenesis. However, the mutation of a single protooncogene or tumor suppressor gene is usually not sufficient to cause neoplastic growth. Tumor progression depends on secondary events which arise during cell proliferation. The genetic targets for these secondary events would be expected to depend on the initiating event as well as developmental and tissue-specific factors regulating cell proliferation. Additional steps involving angiogenesis, invasive growth, and metastasis generate more serious life-threatening malignant disease . Although the “cast of characters” involved in these processes is large and growing, our understanding of the process is complicated by the large number of genes involved, developmental and tissue-specific differences in growth regulation, and the stochastic nature of the process.
KeywordsSpot Intensity Simple Sequence Length Polymorphism Restriction Landmark Genomic Scanning Simple Sequence Length Polymorphism Restriction Landmark Genomic Scanning Profile
Unable to display preview. Download preview PDF.
- 16.Ohsumi T, Okazaki Y, Okuizumi H, Shibata K, Hanami T, Mizuno Y, Takahara T, Sasaki N, Ueda M, Muramatsu M, Kerns KA, Chapman VM, Held WA, Hayashizaki Y (1995) Loss of hetrozygosity in chromosome 1, 5, 7 and 13 in mouse hepatoma detected by systematic genome-wide scanning using RLGS genetic map. Biochem Biophys Res Commun 212:632–639PubMedCrossRefGoogle Scholar
- 17.Dietrich WF, Miller J, Steen R, Merchant MA, Damron-Boles D, Husain Z, Dredge R, Daly MJ, Ingalls KA, O’Connor TJ, Evans CA, DeAngelis MM, Levinson DM, Kruglyak L, Goodman N, Copeland NG, Jenkins NA, Hawkins TL, Stein L, Page DC, Lander ES (1996) A comprehensive genetic map of the mouse genome. Nature 380:149–152PubMedCrossRefGoogle Scholar
- 20.Hayashizaki Y, Hirotsune S, Okazaki Y, Hatada I, Shibata H, Kawai J, Hirose K, Watanabe S, Fushiki S, Wada S, Sugimoto T, Kobayakawa K, Kawara T, Katsuki M, Sibuya T, Mukai T (1993) Restriction landmark genomic scanning method and its various applications. Electrophoresis 14:251–258PubMedCrossRefGoogle Scholar
- 24.Hayashizaki Y, Hirotsune S, Okazaki Y, Shibata H, Akasako A, Muramatsu M, Kawai J, Hirasawa T, Watanabe S, Shiroishi T, Moriwaki K, Taylor BA, Matsuda Y, Elliott RW, Manly KF, Chapman VM (1994) A genetic linkage map of the mouse using Restriction Landmark Genomic Scanning (RLGS). Genetics 138:1207–1238PubMedGoogle Scholar
- 27.Schumacher P, Held WA, Yang D, Biempica L, Rogler CE (1991) Selective amplification of periportal transitional cells precedes formation of hepatocellular carcinoma in SV40 large tag transgenic mice. Am J Pathol 139:231–241Google Scholar
- 28.Akama TO, Okazaki Y, Ito M, Okuizumi H, Konno H, Muramatsu M, Plass C, Held WA, Hayashizaki Y (1997) RLGS-M based genome-wide scanning of mouse liver tumors for alterations in DNA methylation status. Cancer Res, in pressGoogle Scholar