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Protocols for RLGS Gel Production

  • Yasushi Okazaki
  • Hisato Okuizumi
  • Nobuya Sasaki
  • Shuji Takada
  • Tokuei Takahara
  • Yoshihide Hayashizaki
Part of the Springer Lab Manuals book series (SLM)

Abstract

RLGS can be performed using various enzyme combinations (restriction enzymes EL-EB-EC). The labeling method must be chosen according to the shape of the recognition site of enzyme EL. In addition, the reaction buffers used in each process should be changed, accompanied by variation of the enzyme. Here, protocols for two good representative enzyme combinations, combination 1 (NotI-PvuII-PstI) and combination 2 (PacI-EcoRV-MboI), are shown.

Keywords

Restriction Enzyme Digestion Ammonium Persulfate Falcon Tube Dialysis Tube Agarose Electrophoresis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hayashizaki Y, Hirotsune S, Okazaki Y, Muramatsu M, Asakawa J (1995) Restriction landmark genomic scanning (RLGS), Molecular Biology and Biotechnology. VCH Weinheim, pp 813–817Google Scholar
  2. 2.
    Hayashizaki Y, Hirotsune S, Okazaki Y, Muramatsu M, Asakawa J. Restriction landmark genomic scanning (RLGS), Encyclopedia of Molecular Biology, vol 6. VCH, Weinheim (in press)Google Scholar
  3. 3.
    Okazaki Y, Okuizumi S, Sasaki N, Ohsumi T, Kuromitsu J, Kataoka H, Muramatsu M, Iwadate A, Hirota N, Kitajima M, Plass C, Chapman VM, Hayashizaki Y (1994) A genetic linkage map of the mouse using an expanded production system of restriction landmark genomic scanning (RLGS Ver. 1.8). Biochem Biophys Res Commun 205:1922–1929PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 1997

Authors and Affiliations

  • Yasushi Okazaki
  • Hisato Okuizumi
  • Nobuya Sasaki
  • Shuji Takada
  • Tokuei Takahara
  • Yoshihide Hayashizaki

There are no affiliations available

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