Abstract
Direct endlabeling followed by electrophoretic separation of mammalian genomic DNA has been very difficult to perform because of its high complexity. However, three significant breakthroughs have led to the development of RLGS. First, effective restriction endonuclease for genomic analysis has been discovered. Restriction enzymes which recognize 4- or 6-base pair (bp) sequences, conventionally used produce so many DNA fragments that these fragments cannot be separated as discrete signals by electroporesis. Recently, various enzymes have been discovered which recognize 8-bp or rare sequences and produce an appropriate number of DNA fragments for analysis.
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References
Hatada I, Hayashizaki Y, Hirotsune S, Komatsubara K, Mukai T (1991) A genomic scanning method for higher organisms using restriction sites as landmarks on the genome. Proc Natl Acad Sci USA 88:9523–9527
Hayashizaki Y, Hirotsune S, Okazaki Y, Hatada I, Shibata H, Kawai J, Hirose K, Watanabe S, Fushiki S, Wada S, Sugimoto T, Kobayakawa K, Kawara T, Katsuki M, Sibuya T, Mukai T (1993) Restriction landmark genomic scanning and its various applications. Electrophoresis 14:251–258
Okazaki Y, Okuizumi H, Sasaki N, Ohsumi T, Kuromitsu J, Kataoka H, Muramatsu M, Iwadate A, Hirota N, Kitajima M, Plass C, Chapman VM, Hayashizaki Y (1994) A genetic linkage map of the mouse using an expanded production system of restriction landmark genomic scanning (RLGS Ver. 1.8). Biochem Biophys Res Commun 205:1922–1929
Lindsay S, Bird AP (1987) Use of restriction enzyme to detect potential gene sequences in mammalian DNA. Nature 327:336–338
Okuizumi H, Okazaki Y, Sasaki N, Muramatsu M, Nakashima K, Fan K, Tano H, Ohba K, Hayashizaki Y (1994) Application of the RLGS method to large-size genomes using a restriction trapper. DNA Res 1:99–102
Hirotsune S, Shibata H, Okazaki Y, Sugino H, Imoto H, Sasaki N, Hirose K, Okuizumi H, Muramatsu M, Plass C, Chapman VM, Miyamoto C, Tamatsukuri S, Furuichi Y, Hayashizaki Y (1993) Molecular cloning of polymorphic markers on RLGS gel using the spot target cloning method. Biochem Biophys Res Commun 194:1406–1412
Ohsumi T, Okazaki Y, Shibata H, Hirotsune S, Muramastsu M, Suzuki H, Taga C, Watanabe S, Hayashizaki Y (1995) RLGS spot cloning method. Electrophoresis 16:203–209
Okazaki Y, Okuizumi H, Sasaki N, Ohsumi T, Kuromitsu J, Hirota N, Muramatsu M, Hayashizaki Y (1995) A multiplex production systme of RLGS gels’ a new version of restriction landmark genomic scanning method (RLGS Ver.1.8). Electrophoresis 16:197–202
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© 1997 Springer Japan
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Hayashizaki, Y. (1997). Principle of RLGS. In: Hayashizaki, Y., Watanabe, S. (eds) Restriction Landmark Genomic Scanning (RLGS). Springer Lab Manuals. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67953-0_2
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DOI: https://doi.org/10.1007/978-4-431-67953-0_2
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68521-0
Online ISBN: 978-4-431-67953-0
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