Principle of RLGS
Direct endlabeling followed by electrophoretic separation of mammalian genomic DNA has been very difficult to perform because of its high complexity. However, three significant breakthroughs have led to the development of RLGS. First, effective restriction endonuclease for genomic analysis has been discovered. Restriction enzymes which recognize 4- or 6-base pair (bp) sequences, conventionally used produce so many DNA fragments that these fragments cannot be separated as discrete signals by electroporesis. Recently, various enzymes have been discovered which recognize 8-bp or rare sequences and produce an appropriate number of DNA fragments for analysis.
KeywordsRestriction Enzyme Rare Sequence Restriction Landmark Genomic Scanning Restriction Trapper Transcription Regulatory Region
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