Advertisement

Targeting Chemotherapy of Peritoneally Disseminated Gastric Cancer with Monoclonal Antibody-Drug Conjugates

  • Toshiharu Yamaguchi
  • Kazuma Okamoto
  • Toshio Takahashi
  • Kazuya Kitamura
  • Eigo Otsuji
  • Hiroki Taniguchi
  • Chohei Sakakura
  • Morio Shirasu
  • Hiroomi Matsumura
  • Kazuhito Yamamoto
  • Tomoki Nishida
  • Shinichiro Kobayashi
  • Hisakazu Yamagishi

Abstract

Monoclonal antibody A7 was produced by a hybridoma generated by the fusion of splenocytes from a mouse immunized against human colon carcinoma cells and murine myeloma P3.X63.Ag8.653 cells by Kotanagi et al. [1]. A7 is known to react selectively with human adenocarcinoma and to recognize a 45000-dalton glycoprotein on the cell surface [2]. This molecule had been proved to be expressed on the cell surface and rarely shed into the bloodstream or culture medium [3]. Such characteristics are favorable for application of A7 as a carrier of drugs and radioisotopes for immunotargeting chemotherapy or radio-immunoimaging of cancer. The immunoconjugate A7-NCS composed of A7 and neo-carcinostatin (NCS) was developed by Takahashi et al. [4]. In vitro and in vivo experimental studies showed the anticancer activity of A7-NCS to be superior to that of an aqueous solution of NCS against colon cancer. Clinical application of A7-NCS for colorectal cancer was reported in 1988 and 1993 [5].

Keywords

Nude Mouse Tumor Nodule MKN45 Cell Human Colon Carcinoma Cell Gastric Carcinoma Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kotanagi H, Takahashi T, Masuko T, Hashimoto Y, Koyama K (1986) A monoclonal antibody against human colon cancers. Tohoku J Exp Med 148:353–360PubMedCrossRefGoogle Scholar
  2. 2.
    Kitamura K, Takahashi T, Yamaguchi T, Yokota T, Noguchi A, Amagai T, Imanishi J (1989) Immunochemical characterization of the antigen recognized by the murine monoclonal antibody A7 against human colorectal cancer. Tohoku J Exp Med 157:83–89PubMedCrossRefGoogle Scholar
  3. 3.
    Otsuji E, Yamaguchi T, Yamaguchi N, Koyama K, Imanishi J, Yamaoka N, Takahashi T (1992) Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines. Sugery Today 22:351–356CrossRefGoogle Scholar
  4. 4.
    Takahashi T, Yamaguchi T, Kitamura K, Suzuyama H, Honda M, Yokota T, Kotanagi H, Takahashi M, Hashimoto Y (1988) Clinical application of monoclonal antibody-drug conjugates for immunotargeting chemotherapy of colorectal carcinoma. Cancer 61:881–888PubMedCrossRefGoogle Scholar
  5. 5.
    Takahashi T, Yamaguchi T, Kitamura K, Noguchi A, Honda M, Otsuji E (1993) Follow-up study of patients treated with monoclonal antibody-drug conjugate: report of 77 cases with colorectal cancer. Jpn J Cancer Res 84:976–981PubMedCrossRefGoogle Scholar
  6. 6.
    Yamaoka N, Yamaguchi T, Otsuji E, Kato M, Kotani T, Kitamura K, Takahshi T (1994) In vitro reactivity and in vivo biodistribution of the monoclonal antibody A7 using human gastric carcinoma cell lines. Br J Cancer 70:405–408PubMedCrossRefGoogle Scholar
  7. 7.
    Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRefGoogle Scholar
  8. 8.
    Ishiyama M, Shiga M, Sasamoto K, Mizoguchi M, He PG (1993) A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem Pharm Bull (Tokyo) 41:1118–1122CrossRefGoogle Scholar
  9. 9.
    Hagiwara A, Takahashi T, Lee R, Ueda M, Takeda M, Itoh T (1987) Chemotherapy for carcinomatous peritonitis and pleuritis with MMC-CH, mitomycin C adsorbed on activated carbon particles, clinical trials. Cancer 59:245–251PubMedCrossRefGoogle Scholar
  10. 10.
    Ehrlich P (1900) On immunity with special reference to cell life. Proc R Soc Lond 66:424–448Google Scholar
  11. 11.
    Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 259:495–497CrossRefGoogle Scholar
  12. 12.
    Kitamura K, Takahashi T, Noguchi A, Takashina K, Tsurumi H, Yamaguchi T (1990) Binding, internalization and the cytotoxicity of monoclonal antibody A7-neocarzinostatin conjugates (A7-NCS) in target cells. Tohoku J Exp Med 161:199–207PubMedCrossRefGoogle Scholar
  13. 13.
    Yamaguchi T, Tsurumi H, Kitamura K, Otsuji E, Miyagaki T, Kotani T, Takahashi T (1993) Production, binding and cytotoxicity of human/mouse chimeric monoclonal antibody-neocarzinostatin conjugate. Jpn J Cancer Res 84:1190–1194PubMedCrossRefGoogle Scholar
  14. 14.
    Yamaguchi T, Tsurumi H, Kotani T, Yamaoka N, Otsuji E, Kitamura K, Takahashi T (1994) In vivo efficacy of neo-carzinostatin coupled with Fab human/mouse chimeric monoclonal antibody A7 against human colorectal cancer. Jpn J Cancer Res 85:167–171PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 1999

Authors and Affiliations

  • Toshiharu Yamaguchi
    • 1
  • Kazuma Okamoto
    • 1
  • Toshio Takahashi
    • 2
  • Kazuya Kitamura
    • 1
  • Eigo Otsuji
    • 1
  • Hiroki Taniguchi
    • 1
  • Chohei Sakakura
    • 1
  • Morio Shirasu
    • 1
  • Hiroomi Matsumura
    • 1
  • Kazuhito Yamamoto
    • 1
  • Tomoki Nishida
    • 1
  • Shinichiro Kobayashi
    • 1
  • Hisakazu Yamagishi
    • 1
  1. 1.First Department of SurgeryKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Tokyo Metropolitan Komagome HospitalTokyoJapan

Personalised recommendations