Targeting Chemotherapy of Peritoneally Disseminated Gastric Cancer with Monoclonal Antibody-Drug Conjugates
Monoclonal antibody A7 was produced by a hybridoma generated by the fusion of splenocytes from a mouse immunized against human colon carcinoma cells and murine myeloma P3.X63.Ag8.653 cells by Kotanagi et al. . A7 is known to react selectively with human adenocarcinoma and to recognize a 45000-dalton glycoprotein on the cell surface . This molecule had been proved to be expressed on the cell surface and rarely shed into the bloodstream or culture medium . Such characteristics are favorable for application of A7 as a carrier of drugs and radioisotopes for immunotargeting chemotherapy or radio-immunoimaging of cancer. The immunoconjugate A7-NCS composed of A7 and neo-carcinostatin (NCS) was developed by Takahashi et al. . In vitro and in vivo experimental studies showed the anticancer activity of A7-NCS to be superior to that of an aqueous solution of NCS against colon cancer. Clinical application of A7-NCS for colorectal cancer was reported in 1988 and 1993 .
KeywordsNude Mouse Tumor Nodule MKN45 Cell Human Colon Carcinoma Cell Gastric Carcinoma Cell Line
Unable to display preview. Download preview PDF.
- 10.Ehrlich P (1900) On immunity with special reference to cell life. Proc R Soc Lond 66:424–448Google Scholar