In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe

  • Hirotaka Matsumi
  • Tetsu Yano
Part of the Springer Lab Manuals book series (SLM)


As has been shown previously (Koji et al. 1988; 1990; 1996), nonradioactively labeled probes are widely used for in situ hybridization because they have several advantages compared to radioactive ones.


Dextran Sulfate Moist Chamber Hybridization Mixture Yeast tRNA Chromogen Solution 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams J.C. (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29: 775PubMedCrossRefGoogle Scholar
  2. Koji T, Nakane PK (1990) Localization in situ of specific mRNA using thymine-thymine dimerized DNA probes. Sensitive and reliable non-radioactive in situ hybridization. Acta Pathol Jpn 40: 793–807PubMedGoogle Scholar
  3. Koji T, Nakane P.K. (1996) Recent advances in molecular histochemical techniques: in situ hybridization and southwestern histochemistry. J Electron Microsc 45: 119–127CrossRefGoogle Scholar
  4. Koji T, Moriuchi T, Nakane P.K. (1988) Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21:187–200CrossRefGoogle Scholar
  5. Hakuno N, Koji T, Yano T, Kobayashi N, Tsutsumi O, Taketani Y, Nakane P.K. (1996) Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia. Endocrinology 137: 1938–1948PubMedCrossRefGoogle Scholar
  6. Matsumi H, Yano T, Koji T, Ogura T, Tsutsumi O, Taketani Y, Esumi H (1998) Expression and Localization of Inducible Nitric Oxide Synthase in the Rat Ovary: A Possible Involvement of Nitric Oxide in the Follicular Development. Biochem Biophys Res Commun 243: 67–72PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Hirotaka Matsumi
    • 1
  • Tetsu Yano
  1. 1.Department of Obstetrics and Gynecology, Faculty of MedicineUniversity of TokyoTokyoJapan

Personalised recommendations