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In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe

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Book cover Molecular Histochemical Techniques

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

As has been shown previously (Koji et al. 1988; 1990; 1996), nonradioactively labeled probes are widely used for in situ hybridization because they have several advantages compared to radioactive ones.

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References

  • Adams J.C. (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29: 775

    Article  PubMed  CAS  Google Scholar 

  • Koji T, Nakane PK (1990) Localization in situ of specific mRNA using thymine-thymine dimerized DNA probes. Sensitive and reliable non-radioactive in situ hybridization. Acta Pathol Jpn 40: 793–807

    PubMed  CAS  Google Scholar 

  • Koji T, Nakane P.K. (1996) Recent advances in molecular histochemical techniques: in situ hybridization and southwestern histochemistry. J Electron Microsc 45: 119–127

    Article  CAS  Google Scholar 

  • Koji T, Moriuchi T, Nakane P.K. (1988) Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21:187–200

    Article  CAS  Google Scholar 

  • Hakuno N, Koji T, Yano T, Kobayashi N, Tsutsumi O, Taketani Y, Nakane P.K. (1996) Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia. Endocrinology 137: 1938–1948

    Article  PubMed  CAS  Google Scholar 

  • Matsumi H, Yano T, Koji T, Ogura T, Tsutsumi O, Taketani Y, Esumi H (1998) Expression and Localization of Inducible Nitric Oxide Synthase in the Rat Ovary: A Possible Involvement of Nitric Oxide in the Follicular Development. Biochem Biophys Res Commun 243: 67–72

    Article  PubMed  CAS  Google Scholar 

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© 2000 Springer Japan

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Matsumi, H., Yano, T. (2000). In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe. In: Koji, T. (eds) Molecular Histochemical Techniques. Springer Lab Manuals. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67915-8_9

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  • DOI: https://doi.org/10.1007/978-4-431-67915-8_9

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68520-3

  • Online ISBN: 978-4-431-67915-8

  • eBook Packages: Springer Book Archive

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