In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe
Part of the Springer Lab Manuals book series (SLM)
As has been shown previously (Koji et al. 1988; 1990; 1996), nonradioactively labeled probes are widely used for in situ hybridization because they have several advantages compared to radioactive ones.
KeywordsDextran Sulfate Moist Chamber Hybridization Mixture Yeast tRNA Chromogen Solution
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- Koji T, Moriuchi T, Nakane P.K. (1988) Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21:187–200CrossRefGoogle Scholar
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