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In Situ Hybridization for RNA: Radioactive RNA Probe — A Protocol for Practice With a Kit

  • Hidefumi Yoshioka
  • Sumihare Noji
Chapter
Part of the Springer Lab Manuals book series (SLM)

Abstract

Recently, nonradioactive probe has often been used to detect RNAs in sectioned tissues by in situ hybridization (ISH) method. Although color-based detection systems with nonradioactive probe is becoming popular in ISH methods, the method with radioactive (RI) probes is still the most sensitive way to detect RNAs in the tissues. Thus, if a given mRNA cannot be detected in tissues with color-based detection methods, although it is confirmed to be present by PCR, the final way to detect the mRNA is to use the ISH method with an RI probe. Processes of specimen preparation, pre-treatments, and hybridization are similar in either radioactive or nonradioactive methods. A major difference between them is the detection method, i.e., autoradiography is used for detection of an RI probe.

Keywords

Bovine Serum Albumin Prehybridization Solution Incorporation Ratio Granular Convoluted Tubule Black Tape 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Noji S., Takahashi N., Nohno T., Koyama E., Yamaai T., Muramatsu M., and Taniguchi S (1990) Expression pattern of the homeobox gene Hox-3.5 during mouse development, as revealed by a simplified in situ hybridization method. Acta Histochem. Cytochem. 23:353–366.CrossRefGoogle Scholar
  2. Noji S. (1997) A practical use of a kit for in situ hybridization with tissue section. In: Immunostaining and In situ hybridization (Yodosha The Protocol Series). Yodosha, Tokyo, pp58–79Google Scholar
  3. Angerer L.M. and Angerer R.C. (1992) In situ hybridization to cellular RNA with radiolabelled RNA probes. In: Wilkinson D.G. (ed) In situ hybridization, IRL press, Oxford New York Tokyo, pp15–32Google Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Hidefumi Yoshioka
    • 1
  • Sumihare Noji
    • 1
  1. 1.Department of Biological Science and Technology, Faculty of EngineeringThe University of TokushimaTokushima CityJapan

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