In Situ Hybridization for RNA: Radioactive DNA Probe
In situ hybridization (ISH) was first described in 1969 by Pardue and Gall. Tritium labeled nucleic acid was first used and detected via exposure to a nuclear track emulsion. Though ISH has been improved over the years, it still takes a long time to obtain fine and precise results especially when using tritium labeled probes as compared to hours of those non-radioacitve probes. The other potential labels such as 125I and 32P for ISH were recognized in the early 1970s. The limitations of radioactive isotopic detection for ISH were recognized from an early stage. Today in vitro biochemical labeling of purified nucleic acids is universally employed and in the future it seems that chemically synthesized oligonucleotides will be used.
KeywordsDextran Sulfate Nick Translation Rubber Cement Standard Sodium Citrate Nuclear Track Emulsion
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