In Situ Hybridization for RNA: Radioactive DNA Probe

  • Takashi Sawai
  • Miwa Uzuki
Part of the Springer Lab Manuals book series (SLM)


In situ hybridization (ISH) was first described in 1969 by Pardue and Gall. Tritium labeled nucleic acid was first used and detected via exposure to a nuclear track emulsion. Though ISH has been improved over the years, it still takes a long time to obtain fine and precise results especially when using tritium labeled probes as compared to hours of those non-radioacitve probes. The other potential labels such as 125I and 32P for ISH were recognized in the early 1970s. The limitations of radioactive isotopic detection for ISH were recognized from an early stage. Today in vitro biochemical labeling of purified nucleic acids is universally employed and in the future it seems that chemically synthesized oligonucleotides will be used.


Dextran Sulfate Nick Translation Rubber Cement Standard Sodium Citrate Nuclear Track Emulsion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brady M.A.W and Finlan M (1990) Radioactive labels: autoradiography and choice of emulsions for in situ hybridization. In In situ hybridization-principles and practice-(eds. J.M. Polak and JO’ D. McGEE), Oxford University Press Oxford-New York-TokyoGoogle Scholar
  2. Hafen E, Levine M, Garber L.G. and Gehring W.J. (l983) An improved in stiu hybridization method for the detection of cellular RNAs in Drosophilia tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex. The EMBO J 2:617–23Google Scholar
  3. Hayashi M, Ninomiya Y, Parson J et al (l986) Differential localization of mRNAs of collagen types land 11 in chick fibroblasts, chondrocytes, and corneal cells by in situ hybridization using cDNA probes. J Cell Biol102: 2302–9CrossRefGoogle Scholar
  4. Pardue M.L. and Gall J.G. (l969) Molecular hybridization of radioactive DNA to the DNA of cytological preparations. Proc. Natl Acad Sci 70: 1860–4Google Scholar
  5. Sawai T and Uzuki M (1996) In situ hybridization by radioactive cDNA probes-practice and application-In Histochemistry i n 1996. ed by K Nakane, PP129–136,Gakusai Kikaku, TokyoGoogle Scholar
  6. Sawai T, Uzuki M, Harris Jr. E.D., Kurkinnen M, Trelstad R.L. and Hayashi M (1996) In situ hybridization of stromelysin mRNA in the synovial biopsies from rheumatoid arthritis. Tohoku J. Exp. Med. 178: 315–330PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Takashi Sawai
    • 1
  • Miwa Uzuki
  1. 1.Dept. of PathologyIwate Medical UniversityMorioka-CityJapan

Personalised recommendations