In Situ Hybridization for DNA: Fluorescent Probe
Fluorescence in situ hybridization (FISH) with chromosomespecific probes is one of the non-isotopic in situ hybridization methods that opens up several new areas of cytogenetic investigation by allowing visual determination of the presence and normality of specific genetic sequences in single metaphase or interphase cells (Pinkel 1986a, 1986b). With this approach, the genetic loci to be analyzed are made microscopically visible in single cells using in situ hybridization with nucleic acid probes specific to these loci. The DNA in target cells is made as a single strand and incubated with single-stranded fluoro chromedirectly- labeled or chemically modified DNA probe under conditions where the probe will anneal only with DNA sequences to which it has high DNA sequence homology. The bound chemically modified probe is made visible by immunofluorescent detection methods. This technique is often called standard FISH.
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