Introduction to the Detection of Specific DNA and RNA Sequences
In situ hybridization was originally developed by Gall and Pardue (1969) and John et al. (1969) as a method to localize specific DNA sequences in cytological preparation. Afterwards, the method was used as a powerful technique to detect the locus of a specific gene in chromosomes or the sites of viral DNA. In the late 1970s, Brahic and Haase (1978) revealed that the same principle is applicable to localize specific RNA in cell and tissue preparations. In keeping pace with the burst prosperity of molecular cloning fields, in situ hybridization has progressed and now has turned out to be one of the most essential techniques in cell biology and developmental biology. In this chapter, I will try to overview the methodological aspects of in situ hybridization, particularly focusing on the detection of specific RNA in tissue sections, in which we have been actively engaged for more than 15 years (Koji and Nakane 1990, 1996).
KeywordsKeratinocyte Growth Factor Protease Digestion Probe Nucleic Acid Estrogen Receptor mRNA Catalyze Signal Amplification
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