In Situ Nick End-labeling:Electron Microscopical

  • Yoshinori Otsuki
  • Yuko Ito
Part of the Springer Lab Manuals book series (SLM)


Transmission electron microscopy (TEM) gives us much morphological proposed by Kerr et al. (1972) was based on several characteristic ultrastructural findings. Agarose gel electrophoresis using extracted DNA is preferentially used in the molecular-biological studies dealing with apoptosis and reveals the apoptotic nuclear changes such as nucleosomal DNA fragmentation i.e., DNA ladder (Arends et al. 1990). In addition to TEM and agarose gel electrophoresis, in situ end labeling of DNA strand breaks (ISEL) technique using light microscopy (LM)( conventional ISEL technique) which labels free 3’-OH ends is widely used for the discrimination between apoptotic cells and necrotic cells. Recently, much interest has been focused on the problems related to the specificity of both the agarose gel electrophoresis of extracted DNA and the conventional ISEL technique in the detection of cell death, although both are far more convenient methods than TEM.


Apoptotic Cell Methyl Green Osaka Medical College Label Density Immunogold Particle 


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Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Yoshinori Otsuki
    • 1
  • Yuko Ito
    • 2
  1. 1.Department of Anatomy and BiologyOsaka Medical CollegeOsakaJapan
  2. 2.Department of Anatomy and BiologyOsaka Medical CollegeOsakaJapan

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