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In Situ Nick End-labeling: Light Microscopical

  • Shigeyoshi Oba
  • Takehiko Koji
Chapter
Part of the Springer Lab Manuals book series (SLM)

Abstract

It has been postulated that in apoptotic cell death, the first biochemical event is single-strand DNA breaks followed by doublestrand DNA cleavage in the linker regions between nucleosomes (Peitsh 1993). TUNEL (Terminal transferase-mediated dUTPbiotin nick end labeling) is a convenient molecular histochemical tool to detect these single-DN A breaks. Briefly described, this tool is based on repeated addition of biotin-dUTP at the 3-hydroxy ends of DNA of tissue by terminal deoxynucleotidyl transferase and detection of these additional biotin-dUTP by HRP labeled anti-biotin antibody. You can use digoxigenin-dUTP or fluorescent conjugated dUTP instead of biotin-dUTP.

Keywords

Terminal Deoxynucleotidyl Transferase Moist Chamber Cobalt Iron Mannheim Biochemical Excessive Background 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adams J.C.; J. Histochem Cytochem 29: 775, 1981PubMedCrossRefGoogle Scholar
  2. Gavieli, Y., Sherman, Y., Ben-Sasson, S.A.; J. Cell Biol., 119:493–501,1992CrossRefGoogle Scholar
  3. Peitsh M.C., Muller C, Tschopp J (1993) DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucl. Acid Res. 21:4206–4209CrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Shigeyoshi Oba
    • 1
  • Takehiko Koji
    • 2
  1. 1.The 2nd Department of Internal Medicine, Faculty of MedicineThe University of TokyoTokyoJapan
  2. 2.Dept. of Histology and Cell BiologyNagasaki UniversityNagasakiJapan

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