In Situ Nick End-labeling: Light Microscopical

  • Shigeyoshi Oba
  • Takehiko Koji
Part of the Springer Lab Manuals book series (SLM)


It has been postulated that in apoptotic cell death, the first biochemical event is single-strand DNA breaks followed by doublestrand DNA cleavage in the linker regions between nucleosomes (Peitsh 1993). TUNEL (Terminal transferase-mediated dUTPbiotin nick end labeling) is a convenient molecular histochemical tool to detect these single-DN A breaks. Briefly described, this tool is based on repeated addition of biotin-dUTP at the 3-hydroxy ends of DNA of tissue by terminal deoxynucleotidyl transferase and detection of these additional biotin-dUTP by HRP labeled anti-biotin antibody. You can use digoxigenin-dUTP or fluorescent conjugated dUTP instead of biotin-dUTP.


Terminal Deoxynucleotidyl Transferase Moist Chamber Cobalt Iron Mannheim Biochemical Excessive Background 


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  2. Gavieli, Y., Sherman, Y., Ben-Sasson, S.A.; J. Cell Biol., 119:493–501,1992CrossRefGoogle Scholar
  3. Peitsh M.C., Muller C, Tschopp J (1993) DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucl. Acid Res. 21:4206–4209CrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Shigeyoshi Oba
    • 1
  • Takehiko Koji
    • 2
  1. 1.The 2nd Department of Internal Medicine, Faculty of MedicineThe University of TokyoTokyoJapan
  2. 2.Dept. of Histology and Cell BiologyNagasaki UniversityNagasakiJapan

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