In Situ Nick End-labeling: Light Microscopical
It has been postulated that in apoptotic cell death, the first biochemical event is single-strand DNA breaks followed by doublestrand DNA cleavage in the linker regions between nucleosomes (Peitsh 1993). TUNEL (Terminal transferase-mediated dUTPbiotin nick end labeling) is a convenient molecular histochemical tool to detect these single-DN A breaks. Briefly described, this tool is based on repeated addition of biotin-dUTP at the 3-hydroxy ends of DNA of tissue by terminal deoxynucleotidyl transferase and detection of these additional biotin-dUTP by HRP labeled anti-biotin antibody. You can use digoxigenin-dUTP or fluorescent conjugated dUTP instead of biotin-dUTP.
KeywordsTerminal Deoxynucleotidyl Transferase Moist Chamber Cobalt Iron Mannheim Biochemical Excessive Background
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