In Situ Nick Translation
Nick (DNA single strand break) have been implicated to play important roles in the regulation of cell proliferation (Ogawa 1980), cell differentiation (Appleby 1977) and cell death (Peitsch 1993). Nick translation detects nicks in DNA by adding nucleotides to 3’-hydroxyl ends in the presence of a template, extending the strand in the 5’ to 3’ direction. (Fig. 1) In situ nick translation (ISNT)(Dawson 1980) is application of nick translation on frozen sections or formalin fixed paraffin embedded tissues.
KeywordsProliferate Cell Nuclear Antigen Human Colorectal Cancer Nick Translation Fresh Freeze Sample Paraffin Embed Sample
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