In Situ Nick Translation

  • Satoshi Hashimoto
  • Takashi Kanematsu
Part of the Springer Lab Manuals book series (SLM)


Nick (DNA single strand break) have been implicated to play important roles in the regulation of cell proliferation (Ogawa 1980), cell differentiation (Appleby 1977) and cell death (Peitsch 1993). Nick translation detects nicks in DNA by adding nucleotides to 3’-hydroxyl ends in the presence of a template, extending the strand in the 5’ to 3’ direction. (Fig. 1) In situ nick translation (ISNT)(Dawson 1980) is application of nick translation on frozen sections or formalin fixed paraffin embedded tissues.


Proliferate Cell Nuclear Antigen Human Colorectal Cancer Nick Translation Fresh Freeze Sample Paraffin Embed Sample 
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Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Satoshi Hashimoto
    • 1
  • Takashi Kanematsu
    • 1
  1. 1.Second Department of SurgeryNagasaki University School of MedicineNagasakiJapan

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