Southwestern Histochemistry

  • Ko Komuta
  • Takashi Kanematsu
  • Takehiko Koji
Part of the Springer Lab Manuals book series (SLM)


Abnormal expression of oncogenes or proto-oncogenes are frequently related with cancer development. The epidermal growth factor receptor (EGFR) gene has been found to be amplified and/ or expressed at high levels in various types of tumor cells including A431 human epidermoid carcinoma cells [1,2,3]. Although the over-production of EGFR protein generally correlates with an elevated level of its mRNA, it does not always accompany EGFR gene amplification [4,5].Therefore, to understand the abnormal nature of cancer growth, the analysis of their gene expression at the transcriptional level as well as the transcript level is required.


Epidermal Growth Factor Receptor Epidermal Growth Factor Receptor Gene Epidermal Growth Factor Receptor Protein A431 Tumor Situ Hybridization 


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Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Ko Komuta
    • 1
  • Takashi Kanematsu
    • 1
  • Takehiko Koji
    • 2
  1. 1.Department of Surgery IINagasaki University School of MedicineNagasakiJapan
  2. 2.Department of Histology and Cell BiologyNagasaki University School of MedicineNagasakiJapan

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