Introduction to the Detection of Transcription Regulatory Proteins

  • Takehiko Koji
Part of the Springer Lab Manuals book series (SLM)


Does an increase in the level of a certain mRNA mean the increase in transcriptional activity?


Glucocorticoid Receptor Transcription Regulatory Factor POMC mRNA Glucocorticoid Responsive Element Fresh Freeze Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Beato M (1993) Gene regulation by steroid hormones. Cell 56:335–344CrossRefGoogle Scholar
  2. Curran T, Franza R.B. (1988) Fos and Jun: The AP-1 connection. Cell 55:395–397PubMedCrossRefGoogle Scholar
  3. Evans R.M. (1988) The steroid and thyroid hormone receptor. Science 240:889–895PubMedCrossRefGoogle Scholar
  4. Kerkhoff E, Bister K, Klempnauer K-H (1991) Sequence-specific DNA binding by Myc proteins. Proc Natl Acad Sci USA 88:4323–4327PubMedCrossRefGoogle Scholar
  5. Koji T, Komuta K, Nozawa M, Yamada S, Nakane P.K. (1994) Localization of cyclic adenosine 3’,5’-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry. J Histochem Cytochem 42:1399–1405PubMedCrossRefGoogle Scholar
  6. Koji T, Moriuchi T, Nakane P.K. (1988) Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: Effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21:187–200CrossRefGoogle Scholar
  7. Koji T, Yamada S, Izumi S, Nakane P.K. (1990) Oligo-histochemistry. A new approach to localize DNA-binding proteins. J Histochem Cytochem 38:1052Google Scholar
  8. Koji T, Yamada S, Kayashima K, Nakane P.K. (1992) A new approach to localize glucocorticoid receptor using DNA probe containing glucocorticoid responsive element DNA consensus sequences. Acta Histochem Cytochem 25:681–687CrossRefGoogle Scholar
  9. Meyer T.E., Habener J.F. (1993) Cyclic adenosine 3’,5’-monophosphate response element binding protein (CREB) and related transcription-activating deoxyribonucleic acid-binding proteins. Endocrine Rev 14:269–290Google Scholar
  10. Miskimins W.K., Roberts M, McClelland A, Ruddle F.H. (1985) Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. Proc Natl Acad Sci USA 82:6741–6744PubMedCrossRefGoogle Scholar
  11. Tully D.B., Cidlowski J.A. (1987) pBR322 contains glucocorticoid regulatory element DNA consensus sequences. Biochem Biophys Res Commun 144:1–10PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Takehiko Koji
    • 1
  1. 1.Dept. of Histology and Cell BiologyNagasaki UniversityNagasakiJapan

Personalised recommendations