In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe

  • Haruo Ohtani
Part of the Springer Lab Manuals book series (SLM)


Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.


Moist Chamber Sense Orientation Antisense Orientation Proper Fixation Alkaline Phosphatase Reaction 


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Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Haruo Ohtani
    • 1
  1. 1.Department of PathologyTohoku University School of MedicineSendaiJapan

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