In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe
Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.
KeywordsMoist Chamber Sense Orientation Antisense Orientation Proper Fixation Alkaline Phosphatase Reaction
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