Advertisement

In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer

  • Yoshitaka Hishikawa
  • Masamoto Murakami
  • Masao Kasahara
  • Naofumi Nagasue
Chapter
Part of the Springer Lab Manuals book series (SLM)

Abstract

The method using thymine-thymine (T-T) dimerized DNA as a haptenic probe was previously reported (Koji 1988, 1989, 1990, 1996) and we have elaborated it in the following sections.

Keywords

Antisense Probe Yeast tRNA Deionized Formamide Dimerized Probe Nonradioactive Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams J.C. (1981) Heavy metal intensification of DAB-based HRP reaction products. J Histochem Cytochem 29:775PubMedCrossRefGoogle Scholar
  2. Feavers I.M., McEwan IJ, Liang H, Jost J-P (1989) An estradiol-dependent protein from chicken liver binds single-stranded DNA and RNA. J Bio Chem 264:9114–9117Google Scholar
  3. Franklin W.A., Lo K.M., Haseltine W.A. (1982) J Biol Chem 257:13535–13543PubMedGoogle Scholar
  4. Kimelman D, Kirschner M.W. (1989) An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes. Cell 59:687–696PubMedCrossRefGoogle Scholar
  5. Koji T, Moriuchi T, Nakane P.K. (1988) Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: Effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21:187–200CrossRefGoogle Scholar
  6. Koji T, Nakane P.K. (1990) Localization in situ of specific mRNA using Thymine-Thymine dimerized DNA probes: sensitive and reliable non-radioactive in situ hybridization. Acta Pathol Jpn 40:793–807PubMedGoogle Scholar
  7. Koji T, Nakane P.K. (1996) Recent advances in molecular-histochemical-techniques: in situ hybridization and southwestern histochemistry. J Electron Microsc 45:119–127CrossRefGoogle Scholar
  8. Koji T, Sugawara I, Kimura M et al. (1989) In situ localization of c-myc mRNA in HL-60 cells using non radioactive synthetic oligonucleotide probes. Acta Histochem Cytochem 22:295–307CrossRefGoogle Scholar
  9. Meinkoth J, Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138:267–284PubMedCrossRefGoogle Scholar
  10. Shulte E.K.W., Lyon H.O., Hoyer P.E. (1992) Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry. Histochem J 24:305–310CrossRefGoogle Scholar
  11. Singh L, Jones K.W. (1984) The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucleic Acids Res 12:5627–5638PubMedCrossRefGoogle Scholar
  12. Yoshii A, Koji T, Ohsawa N, Nakane P.K. (1995) In situ localization of ribosomal RNAs is a reliable reference for hybridizable RNA in tissue sections. J Histochem Cytochem 43:321–328PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Yoshitaka Hishikawa
    • 1
  • Masamoto Murakami
    • 2
  • Masao Kasahara
    • 3
  • Naofumi Nagasue
    • 4
  1. 1.Department of Histology and Cell BiologyNagasaki University School of MedicineNagasakiJapan
  2. 2.Department of PathologyFujita Health University School of MedicineToyoake-cityJapan
  3. 3.Department of PathologyFujita Health University School of MedicineToyoake-cityJapan
  4. 4.Second Department of SurgeryShimane Medical UniversityIzumoJapan

Personalised recommendations