In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe

  • Yoshio Kanemitsu
  • Takehiko Koji
Part of the Springer Lab Manuals book series (SLM)


The use of single-stranded cD NA (ss cDNA) is more advantageous in sensitivity than that of double-stranded cD NA (ds cDNA) or synthetic oligonucleotide as a probe for in situ hybridization (ISH) (Sugawara et al. 1990). In this chapter, we present a simple procedure for the production of nonradioactive ss cD NA probe by combining the two-step asymmetric PCR and thyminse-thyminse dimer (T-T dimer) haptenization.


Thermal Controller Vortex Briefly Nonradioactive Probe Optimum Time Parameter Versus Reverse Transcriptase 


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Copyright information

© Springer Japan 2000

Authors and Affiliations

  • Yoshio Kanemitsu
    • 1
  • Takehiko Koji
    • 2
  1. 1.Department of Physiology, Faculty of MedicineKyushu UniversityFukuokaJapan
  2. 2.Department of AnatomyNagasaki University School of MedicineNagasakiJapan

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