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In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe

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Molecular Histochemical Techniques

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

The use of single-stranded cD NA (ss cDNA) is more advantageous in sensitivity than that of double-stranded cD NA (ds cDNA) or synthetic oligonucleotide as a probe for in situ hybridization (ISH) (Sugawara et al. 1990). In this chapter, we present a simple procedure for the production of nonradioactive ss cD NA probe by combining the two-step asymmetric PCR and thyminse-thyminse dimer (T-T dimer) haptenization.

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References

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Author information

Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Yoshio Kanemitsu

  2. Department of Anatomy, Nagasaki University School of Medicine, Sakamoto, Nagasaki, 852, Japan

    Takehiko Koji

Authors
  1. Yoshio Kanemitsu
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  2. Takehiko Koji
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Editor information

Editors and Affiliations

  1. Department of Histology and Cell Biology, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan

    Takehiko Koji Ph.D. (Associate Professor) (Associate Professor)

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© 2000 Springer Japan

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Kanemitsu, Y., Koji, T. (2000). In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe. In: Koji, T. (eds) Molecular Histochemical Techniques. Springer Lab Manuals. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67915-8_10

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  • DOI: https://doi.org/10.1007/978-4-431-67915-8_10

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68520-3

  • Online ISBN: 978-4-431-67915-8

  • eBook Packages: Springer Book Archive

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