In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe
The use of single-stranded cD NA (ss cDNA) is more advantageous in sensitivity than that of double-stranded cD NA (ds cDNA) or synthetic oligonucleotide as a probe for in situ hybridization (ISH) (Sugawara et al. 1990). In this chapter, we present a simple procedure for the production of nonradioactive ss cD NA probe by combining the two-step asymmetric PCR and thyminse-thyminse dimer (T-T dimer) haptenization.
KeywordsThermal Controller Vortex Briefly Nonradioactive Probe Optimum Time Parameter Versus Reverse Transcriptase
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