Abstract
Interferon regulatory factor-1 is an important transcription factor in the interferon system. We have demonstrated that the growth inhibitory effect of type 1 interferons on human hepatoma cells correlates with levels of transcription of the interferon regulatory factor-1 gene. In this study we investigated whether mutations in this gene cause different responses to type 1 interferons. DNA from several human hepatoma cell lines was investigated, and nucleotide sequences of the interferon regulatory factor-1 gene were determined. Several point mutations were detected in intron 8 of the gene, and one was found to cause deletion of BamHI cleavage sites. We found several point mutations in the upstream region of the interferon regulatory factor-1 exon 1, and the luciferase promoter assay demonstrated that these mutations modified its promoter activity. These results suggest that polymorphisms of the interferon regulatory factor-1 gene partially contribute to determining the response of cancer cells to growth inhibitory effect of type 1 interferons.
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© 2001 Springer Japan
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Saito, H. et al. (2001). Growth Inhibition of Hepatoma Cells by Type 1 Interferons: Modification by Mutations in the Interferon Regulatory Factor-1 Gene. In: Asakura, H., Aoyagi, Y., Nakazawa, S. (eds) Trends in Gastroenterology and Hepatology. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67895-3_26
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DOI: https://doi.org/10.1007/978-4-431-67895-3_26
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-67993-6
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