Gene Flow Analysis of Magnolia obovata Thunb. Using Highly Variable Microsatellite Markers

  • Yuji Isagi
  • Tatsuo Kanazashi
Part of the Ecological Studies book series (ECOLSTUD, volume 158)


In Ogawa Forest Reserve (OFR), the tree community consists of a few dominant tree species, such as Quercus serrata, Fagus japonica, and F. crenata, and many subdominant tree species, such as Acer mono, Betula grossa, Carpinus laxiflora, Swida controversa, Ostrya japonica, Prunus verecunda, Sorbus japonica, and Magnolia obovata (Masaki et al. 1992; also see Chapter 6). These latter species seldom occur as dominants in forest ecosystems, and their standing densities of adult individuals are low: a few to several trees per hectare. However, the populations of these tree species persist and have adequate regeneration, and thus contribute to the forest species diversity.


Gene Flow Seed Dispersal Pollen Donor Pollen Movement Pollen Dispersal 
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  1. Adams WT (1992) Gene dispersal within forest tree populations. New For 6:217–240CrossRefGoogle Scholar
  2. Campbell DR (1991) Comparing pollen dispersal and gene flow in a natural population. Evolution 45:1965–1968CrossRefGoogle Scholar
  3. Campbell DR, Waser NM (1989) Variation in pollen flow within and among populations of Ipomopsis aggregata. Evolution 43: 1444–1455CrossRefGoogle Scholar
  4. Chase MR, Moller C, Kesseli R, Bawa KS (1996) Distant gene flow in tropical trees. Nature 383:398–399CrossRefGoogle Scholar
  5. Devlin B, Ellstrand NC (1990) The development and application of a refined method for estimating gene flow from angiosperm patemity analysis. Evolution 44:248–259CrossRefGoogle Scholar
  6. Devlin B, Roeder K, Ellstrand NC (1988) Fractional patemity assignment: theoretical development and comparison to other methods. Theor Appl Genet 76:369–380PubMedCrossRefGoogle Scholar
  7. Dieringer G, Cabrera LR, Lara M, Loya L, Reyes-Castillo P (1999) Beetle pollination and floral thermogenicity in Magnolia tamaulipana (Magnoliaceae). Int J Plant Sci 160:64–71CrossRefGoogle Scholar
  8. Dow BD, Ashley MV (1996) Microsatellite analysis of seed dispersal and parentage of saplings in bur oak, Quercus macrocarpa. Mol Ecol 5:615–627CrossRefGoogle Scholar
  9. Dow BD, Ashley MV (1998) High levels of gene flow in bur oak revealed by patemity analysis using microsatellites. J Heredity 89:62–70CrossRefGoogle Scholar
  10. Ellstrand NC (1992) Gene flow among seed plant populations. New For 6:241–256CrossRefGoogle Scholar
  11. Ellstrand NC, Marshall DL (1985) Interpopulation gene flow by pollen in wild radish, Raphanus sativus. Am Nat 126:606–616CrossRefGoogle Scholar
  12. Ellstrand NC, Devlin B, Marshall DL (1989) Gene flow by pollen into small populations: data from experimental and natural stands of wild radish. Proc Nat Acad Sci USA 86:9044–9047PubMedCrossRefGoogle Scholar
  13. Govindaraju DR (1988a) Relationship between dispersal ability and levels of gene flow in plants. Oikos 52:31–35CrossRefGoogle Scholar
  14. Govindaraju DR (1988b) A note on the relationship between outcrossing rate and gene flow in plants. Heredity 61:401–404CrossRefGoogle Scholar
  15. Hamrick JL (1987) Gene flow and distribution of genetic variation in plant populations. In: Urbanska K (ed) Differentiation patterns in higher plants. Academic, New York, pp 53–67Google Scholar
  16. Hamrick JL, Murawski DA (1990) The breeding structure of tropical tree populations. Plant Species Biol 5:157–165CrossRefGoogle Scholar
  17. Hamrick JL, Godt MJ, Sherman-Broyles SL (1992) Factors influencing levels of genetic diversity in woody plant species. New For 6:95–124CrossRefGoogle Scholar
  18. Harper JL (1977) Population biology of plants. Academic, LondonGoogle Scholar
  19. Hartl DL, Clark AG (1988) Principles of population genetics, 2nd ed. Sinauer, Sunderland, MAGoogle Scholar
  20. Iida S (1996) Quantitative analysis of acom transportation by rodents using magnetic locator. Vegetatio 124:39–43CrossRefGoogle Scholar
  21. Isagi Y, Kanazashi T, Suzuki W, Tanaka H, Abe T (1999) Polymorphic DNA markers for Magnolia obovata Thumb. and their utility in the related species. Mol Ecol 8:698–700PubMedCrossRefGoogle Scholar
  22. Isagi Y, Kanazashi T, Suzuki W, Tanaka H, Abe T (2000) Microsatellite analysis of the regeneration process of Magnolia obovata. Heredity 84: 143–151PubMedCrossRefGoogle Scholar
  23. Ishida K (1996) Beetle pollination of Magnolia praecocissima var. borealis. Plant Species Biol 11: 199–206CrossRefGoogle Scholar
  24. Ishida K, Nakamura K (1997) Outcrossing rate and inbreeding depression in natural populations of Magnolia hypoleuca. Am J Bot Abstr 84:121Google Scholar
  25. Jensen TS (1985) Seed-seed predator interactions of European beech, Fagus silvatica, and forest rodents, Clethrionomys glareolus and Apodemus flavicollis. Oikos 44: 149–156CrossRefGoogle Scholar
  26. Johnson WC, Adkisson CA (1985) Dispersal of beech nuts by blue jays in fragmented landscapes. Am Midi Nat 113:319–324CrossRefGoogle Scholar
  27. Kameyama Y, Isagi Y, Nakagoshi N (2001) Patterns and levels of gene flow in Rhododendron metternichii var. hondoense revea1ed by microsatellite analysis. Mol Ecol 10:205–216PubMedCrossRefGoogle Scholar
  28. Karron JD, Tucker R, Thumser NN, Reinartz JA (1995) Comparison of pollinator flight movements and gene dispersal patterns in Mimulus ringens. Heredity 75:612–617CrossRefGoogle Scholar
  29. Kikuzawa K, Mizui N (1990) Flowering and fruiting phenology of Magnolia hypoleuca. Plant Species Biol 5:255–261CrossRefGoogle Scholar
  30. Krauss SL (1994) Restricted gene flow within the morpho1ogically complex species Persoonia mollis (Proteaceae): contrasting evidence from the mating system and pollen dispersal. Heredity 73:142–154CrossRefGoogle Scholar
  31. Levin DA (1981) Dispersal versus gene flow in plants. Ann Mo Bot Gard 68:233–253CrossRefGoogle Scholar
  32. Levin DA, Kerster HW (1974) Gene flow in seed plants. Evol Biol 7: 139–220Google Scholar
  33. Masaki T, Suzuki W, Niiyama K, Iida S, Tanaka H, Nakashizuka T (1992) Community structure of a species-rich temperate forest, Ogawa Forest Reserve, central Japan. Vegetatio 98:97–111CrossRefGoogle Scholar
  34. Meagher TR (1986) Analysis of patemity within a natural population of Chamaelirium luteum. 1. Identification of most-likely male parents. Am Nat 128:199–215CrossRefGoogle Scholar
  35. Milligan B (1992) Plant DNA isolation. In: Hoezei, AR (ed) Molecular genetic analysis of populations: a practical approach. IRL Press, Oxford, pp 59–88Google Scholar
  36. Milligan BG, McMurray CK (1993) Dominant vs co dominant genetic markers in the estimation of male mating success. Mol Ecol 2:275–283CrossRefGoogle Scholar
  37. Miyazaki Y, Isagi Y (2000) Pollen flow and intrapopulation genetic structure of Heloniopsis orientalis On forest floor determined using microsatellite markers. Theor Appl Genet 101:718–723CrossRefGoogle Scholar
  38. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Nat Acad Sci USA 70:3321–3323PubMedCrossRefGoogle Scholar
  39. Ramsey MW (1988) Differences in pollinator effectiveness of birds and insects visiting Banksia menziesii (Proteaceae). Oecologia 76: 119–124Google Scholar
  40. Rasmussen IR, Brodsgaad B (1992) Gene flow inferred from seed dispersal and pollinator behavior compared to DNA analysis of restriction site variation in a patchy population of Lotus corniculatus L. Oecologia 89:277–283Google Scholar
  41. Roeder K, Devlin B, Lindsay BG (1989) App1ication of maximum-like1ihood methods to population genetic data for the estimation ofindividual fertilities. Biometrics 45:363–379CrossRefGoogle Scholar
  42. Schaal BA (1980) Measurement of gene flow in Lupinus texensis. Nature 284:450–451CrossRefGoogle Scholar
  43. Schmitt J (1980) Pollinator foraging behavior and gene dispersal in Senecio (Compositae). Evolution 34:934–943CrossRefGoogle Scholar
  44. Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236:787–792PubMedCrossRefGoogle Scholar
  45. Smouse PE, Meagher TR (1994) Genetic analysis of male reproductive contributions in Chamaelirium luteum (L.) Gray (Liliaceae). Genetics 136:313–322PubMedGoogle Scholar
  46. Sork VL (1984) Examination of seed dispersal and survival in red oak, Quercus rubra (Fagaceae), using metal-tagged acoms. Ecology 65: 1020–1022CrossRefGoogle Scholar
  47. Streiff R, Labbe T, Bacielieri R, Steinkellner H, Glössl J, Kremer A (1998). Within population genetic structure in Quercus robur L. and Quercus petraea (Matt.) Liebl. Assessed within isozymes and microsatellites. Mol Ecol 7:317–328CrossRefGoogle Scholar
  48. Tanaka H, Yahara T (1988) The pollination of Magnolia obovata. In: Kawano S (ed) The world of plants II (in Japanese; title trans1ated by the author). Kenkyusya, Tokyo, p 37Google Scholar
  49. Thien LB (1974) Floral biology of Magnolia. Am J Bot 61:1037–1045CrossRefGoogle Scholar
  50. Waser NM (1982) A comparison of distances flown by different visitors to flowers of the same species. Oecologia 55:251–257CrossRefGoogle Scholar
  51. Waser NM (1993) Population structure, optimal outbreeding, and assortative mating in angiospenns. In: Thomhill NW (ed) The natural history of inbreeding and outbreeding. Univ of Chicago Press, Chicago, pp 173–199Google Scholar
  52. Webb CL (1998) The selection of pollen and seed dispersal in plants. Plant Species Biol 13:57–67CrossRefGoogle Scholar
  53. Yasuda M, Nakagoshi N, Nehira K (1991) Examination of the spool-and-line method as a quantitative technique to investigate seed dispersal by rodents. Jpn J Ecol 41:257–262Google Scholar
  54. Yumoto T, Noma N, Maruhashi T (1998) Cheek-pouch dispersal of seeds by Japanese monkeys (Macaca fuscata yakui) on Yakushima Island, Japan. Primates 39:325–338CrossRefGoogle Scholar

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© Springer Japan 2002

Authors and Affiliations

  • Yuji Isagi
  • Tatsuo Kanazashi

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