Advertisement

α-Mannosidase-IIx

  • Tomoya O. Akama
  • Masayoshi Oh-Eda
  • Michiko N. Fukuda

Abstract

Golgi α-mannosidase II (MII) is a processing enzyme involved in N-glycan biosynthesis (Tulsiani et al. 1982b; Moremen and Touster 1985; Moremen et al. 1994). Mil- related enzyme, α-mannosidase IIx (MX), was identified in the human genome; and subsequently, MX cDNA was cloned (Misago et al. 1995).

Keywords

Oligosaccharide Substrate Golgi Mannosidase Man2A2 Gene Single GlcNAc Residue Golgi Membrane Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Misago M, Liao Y-F, Kudo S, Eto S, Mattei M-G, Moremen KW, Fukuda MN (1995) Molecular cloning and expression of cDNAs encoding human a-mannosidase II and a novel a-mannosidase IIx isozyme. Proc Natl Acad Sci USA 92:11766–11770PubMedCrossRefGoogle Scholar
  2. Moremen KW (1989) Isolation of a rat liver Golgi mannosidase II clone by mixed oligonucleotide-primed amplification of cDNA. Proc Natl Acad Sci USA 86:5276–5280PubMedCrossRefGoogle Scholar
  3. Moremen KW, Robbins PW (1991) Isolation, characterization, and expression of cDNAs encoding murine α-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans. J Cell Biol 115:1521–1534PubMedCrossRefGoogle Scholar
  4. Moremen KW, Touster O (1985) Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells. J Biol Chem 260:6654–6662PubMedGoogle Scholar
  5. Moremen KW, Touster O, Robbins PW (1991) Novel purification of the catalytic domain of Golgi α-mannosidase II. characterization and comparison with the intact enzyme. J Biol Chem 266:16876–16885PubMedGoogle Scholar
  6. Moremen KW, Trimble RB, Herscovics A (1994) Glycosidases of asparagine-linked oligosaccharide processing pathway. Glycobiology 4:113–125PubMedCrossRefGoogle Scholar
  7. Ogawa R, Misago M, Fukuda MN, Kudo S, Tsukada J, Morimoto I, Eto S (1996) Structure and transcriptional regulation of human alpha-mannosidase IIx (alpha-mannosidase II isotype) gene. Eur J Biochem 242:446–453PubMedCrossRefGoogle Scholar
  8. Oh-eda M, Nakagawa H, Akama TO, Misago M, Moremen KW, Fukuda MN (2001) Overexpression of the Golgi-localized enzyme α-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway. Eur J Biochem 268:1280–1288PubMedCrossRefGoogle Scholar
  9. Skudlark MD, Orgebin-Crist M-C, Tulsiani RP (1991) Asparagine-linked glycoprotein biosynthesis in rat epididymis; presence of a mannosidase II-like enzyme. Biochem J 277:213–221Google Scholar
  10. Tropea JE, Kaushal GP, Pastuszak I, Mitchell M, Aoyagi T, Molyneux RJ, Elbein AD (1990) Mannostatin A, a new glycoprotein-processing inhibitor. Biochemistry 29:10062–10069PubMedCrossRefGoogle Scholar
  11. Tulsiani DRP, Harris TM, Touster O (1982a) Swainsonine inhibits the biosynthesis of complex glycoproteins by inhibition of Golgi mannosidase II. J Biol Chem 257:7936–7939PubMedGoogle Scholar
  12. Tulsiani DR, Hubbard SC, Robbins PW, Touster O (1982b) Alpha-D-mannosidases of rat liver Golgi membranes: mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases la and Ib are the enzymes converting Man9 precursors to Man5 intermediates. J Biol Chem 257:3660–3668PubMedGoogle Scholar
  13. Tulsiani DRP, Skudlarek MD, Orgebin-Crist M-C (1989) Novel alpha-D-mannosidase of rat sperm plasma membranes; characterization and potential role in sperm-egg interactions. J Cell Biol 109:1257–1267PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Tomoya O. Akama
    • 1
  • Masayoshi Oh-Eda
    • 1
  • Michiko N. Fukuda
    • 1
  1. 1.The Burnham InstituteLa JollaUSA

Personalised recommendations