ALG3 Mannosyltransferase

  • Jonne H. Helenius
  • Claude A. Jakob


N-Linked glycosylation is an essential eukaryotic protein modification. An oligosaccharide consisting of 14 sugar residues is synthesized at the endoplasmic reticulum (ER) membrane before being transferred to a protein (Kornfeld and Kornfeld 1985; Varki 1996; Burda and Aebi 1999). Dolichol phosphate is used as a lipid carrier for the synthesis, which is initiated by the addition of phospho-GlcNAc forming GlcNAc- pyrophosphate-dolichol (GlcNAc-PP-Dol). This is followed, in order, by additions of another GlcNAc, nine mannose, and three glucose residues. On the cytoplasmic side of the ER, activated sugars (UDP-GlcNAc and GDP-Man) are the donors for the two GlcNAc and first five mannose additions, respectively. The Man5GlcNAc2-PP-Dol is then translocated across the membrane into the ER lumen, where dolichol-phosphate- mannose-dependent mannosyltransferases sequentially add four mannose residues. (For further information on Dol-P-Man, see Chapter 78). The synthesis is completed by the transfer of three glucose residues from Dol-P-Glc to the oligosaccharide. (For a description of Dol-P-Glc-dependent transferases, see Chapter 82). The Glc3Man9GlcNAc2 oligosaccharide is transferred to nascent glycoproteins by the oligosaccharyltransferase complex (see Chapter 83).


Killer Toxin Mannose Residue Endoplasmic Reticulum Lumen ALC3 Gene Dolichol Phosphate 
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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jonne H. Helenius
    • 1
  • Claude A. Jakob
    • 1
  1. 1.Insitute for MicrobiologyETH ZürichZürichSwitzerland

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