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Dolichol Phosphate GlcNAc-1-P Transferase

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Handbook of Glycosyltransferases and Related Genes

Abstract

Asparagine-linked glycosylation is a covalent modification of secretory and integral membrane proteins in eukaryotes that modulates the structure and function of these proteins. Dolichol-linked oligosaccharides are transferred en bloc to selected asparagine residues of nascent polypeptides. Biosynthesis of the dolichol-bound oligosaccharides is initiated by formation of GlcNAc-P-P-dolichol from UDP-GlcNAc and dolichol phosphate. This reaction is catalyzed by the endoplasmic reticulum (ER) residential enzyme UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1- phosphate transferase (GPT). The two substrates, dolichol phosphate and UDP- GlcNAc, can serve other pathways, but the product of GPT activity can only participate in lipid-bound oligosaccharide biosynthesis. Thus, GPT catalyzes the committed step of the dolichol cycle involved in asparagine-linked glycosylation.

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Authors and Affiliations

  1. Center for Hygiene and Medical Microbiology, Philipps-University Marburg, Robert-Koch-Strasse 17, 35037, Marburg, Germany

    Andreas Hübel & Ralph T. Schwarz

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  1. Andreas Hübel
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  2. Ralph T. Schwarz
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Editors and Affiliations

  1. Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) & Koichi Honke M.D., Ph.D. (Associate Professor) (Professor) &  (Associate Professor)

  2. Glycobiology Program, Cancer Research Institute, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Minoru Fukuda Ph.D. (Professor) (Professor)

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© 2002 Springer Japan

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Hübel, A., Schwarz, R.T. (2002). Dolichol Phosphate GlcNAc-1-P Transferase. In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_80

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  • DOI: https://doi.org/10.1007/978-4-431-67877-9_80

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-67996-7

  • Online ISBN: 978-4-431-67877-9

  • eBook Packages: Springer Book Archive

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