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Dolichol Phosphate GlcNAc-1-P Transferase

  • Andreas Hübel
  • Ralph T. Schwarz

Abstract

Asparagine-linked glycosylation is a covalent modification of secretory and integral membrane proteins in eukaryotes that modulates the structure and function of these proteins. Dolichol-linked oligosaccharides are transferred en bloc to selected asparagine residues of nascent polypeptides. Biosynthesis of the dolichol-bound oligosaccharides is initiated by formation of GlcNAc-P-P-dolichol from UDP-GlcNAc and dolichol phosphate. This reaction is catalyzed by the endoplasmic reticulum (ER) residential enzyme UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1- phosphate transferase (GPT). The two substrates, dolichol phosphate and UDP- GlcNAc, can serve other pathways, but the product of GPT activity can only participate in lipid-bound oligosaccharide biosynthesis. Thus, GPT catalyzes the committed step of the dolichol cycle involved in asparagine-linked glycosylation.

Keywords

ALG7 Gene Negative Regulatory Element Heterologous Complementation Dolichol Phosphate Polyprenyl Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Andreas Hübel
    • 1
  • Ralph T. Schwarz
    • 1
  1. 1.Center for Hygiene and Medical MicrobiologyPhilipps-University MarburgMarburgGermany

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