Dolichol Phosphate GlcNAc-1-P Transferase

  • Andreas Hübel
  • Ralph T. Schwarz


Asparagine-linked glycosylation is a covalent modification of secretory and integral membrane proteins in eukaryotes that modulates the structure and function of these proteins. Dolichol-linked oligosaccharides are transferred en bloc to selected asparagine residues of nascent polypeptides. Biosynthesis of the dolichol-bound oligosaccharides is initiated by formation of GlcNAc-P-P-dolichol from UDP-GlcNAc and dolichol phosphate. This reaction is catalyzed by the endoplasmic reticulum (ER) residential enzyme UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1- phosphate transferase (GPT). The two substrates, dolichol phosphate and UDP- GlcNAc, can serve other pathways, but the product of GPT activity can only participate in lipid-bound oligosaccharide biosynthesis. Thus, GPT catalyzes the committed step of the dolichol cycle involved in asparagine-linked glycosylation.


ALG7 Gene Negative Regulatory Element Heterologous Complementation Dolichol Phosphate Polyprenyl Phosphate 
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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Andreas Hübel
    • 1
  • Ralph T. Schwarz
    • 1
  1. 1.Center for Hygiene and Medical MicrobiologyPhilipps-University MarburgMarburgGermany

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