Abstract
The core backbone of the glycosylphosphatidylinositol (GPI) anchors contains three mannoses, each linked in different bonds. All three mannoses are donated by dolichol- phosphate-mannose. Three dolichol-phosphate-mannose-dependent mannosyltrans- ferases are therefore involved in biosynthesis of the GPI-anchors. PIG-B is necessary for transfer of the third mannose and is most likely α1,2-mannosyltransferase itself. PIG-B cDNA was expression-cloned using a mouse mutant cell line that is defective in transfer of the third mannose to GPI. PIG-B encodes a 554-amino-acid protein expressed in the endoplasmic reticulum (ER), where GPI is assembled. It has multiple hydrophobic regions, some of which may be transmembrane domains.
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Kinoshita, T., Inoue, N. (2002). PIG-B, GPI-Man Transferase III, Man- (Ethanolaminephosphate)Man-GlcN- (Acy1)PI Mannosyltransferase. In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_79
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DOI: https://doi.org/10.1007/978-4-431-67877-9_79
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