Dolichol Phosphate-Mannose Synthase (DPM1 and DPM2)

  • Yoh-Ichi Shimma
  • Yoshifumi Jigami


Dolichol phosphate-mannose synthase (Dol-P-Man synthase), which generates dolichol phosphate-mannose (Dol-P-Man) from GDP-mannose (GDP-Man) and dolichol phosphate (Dol-P), is an essential enzyme for all eukaryotic cells. The Dol- P-Man synthase is located in the endoplasmic reticulum (ER), through two kinds of binding to the membrane depending on the species (Colussi et al. 1997; Maeda et al. 1998). One group comprises Saccharomyces cerevisiae, Ustilago maydis, and Trypanosoma brucei enzymes, which have a C-terminal hydrophobic domain; and the other group includes the human, mouse, Schizosaccharomyces pombe, and Caenorhabditis briggsiae synthases, which lack a C-terminal hydrophobic domain. In the latter group, at least two polypeptides form the Dol-P-Man synthase complex, where the catalytic subunit DPMI binds to the membrane protein DPM2, which has two membrane spanning regions (Maeda et al. 1998). The Dol-P-Man synthesized by Dol-P-Man synthase is used for the five mannose moieties of N-linked oligosaccharide, the first mannose moiety of fungal O-linked oligosaccharide, three mannose moieties of glycosyl phosphatidylinositol (GPI) anchoring, and C-mannosylation (Orlean 1990; Doucey et al. 1998). Recently, a deficiency of the DPM1 gene was found to cause a congenital disorder of glycosylation (CDG) type Ie, giving rise to mental and psychomotor retardation, dysmorphism, and blood coagulation defects.


Schizosaccharomyces Pombe Phosphate Mannose Trypanosoma Brucei Membrane Span Region Lipid Intermediate 
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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Yoh-Ichi Shimma
    • 1
  • Yoshifumi Jigami
    • 1
  1. 1.Institute of Molecular and Cell Biology (IMCB)National Institute of Advanced Industrial Science and Technology (AIST)Tsukuba-city, IbarakiJapan

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