UDP-Gal Transporter-1 and -2
UDP-Gal transporter is a multiple-segment transmembrane protein of the Golgi apparatus that delivers UDP-Gal, synthesized in the cytosol, into the Golgi lumen to provide various Gal transferases with their substrate for the elongation of carbohydrate chains (Kawakita et al. 1998). Gal transferases, whose catalytic sites face the Golgi lumen, cannot add Gal residues to growing carbohydrate chains unless UDP- Gal is supplied through UDP-Gal transporter. Thus, mutant cultured cell lines defective in UDP-Gal transporter were shown to accumulate Glc-Cer instead of lactosylceramide (Taki et al. 1991), and truncated Nl-inked oligosaccharide chains terminated at GlcNAc (Hara et al. 1989).
KeywordsNewcastle Disease Virus Golgi Membrane Mutant Cell Line Newcastle Disease Virus Infection Microsomal Vesicle
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- Ishida N, Yoshioka S, Iida M, Sudo K, Miura N, Aoki K, Kawakita M (1999) Indispensability of transmembrane domains of Golgi UDP-galactose transporter as revealed by analysis of genetic defects in UDP-galactose transporter-deficient murine Had-1 mutant cell lines and construction of deletion mutants. J Biochem 126:1107–1117PubMedCrossRefGoogle Scholar
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