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Heparan Sulfate/Heparin N-Deacetylase/ N-Sulfotransferase-3 and -4

  • Jun-Ichi Aikawa

Abstract

This chapter describes the third and fourth members of HS/heparin N-deacetylase/ N-sulfotransferase (NDST). Refer to Chapters 70 and 71 for information about NDST-1 and NDST-2, respectively. Searching databases, NDST-3 and NDST-4 are discovered in the EST and genomic databases, respectively, as two new homologs to well known NDST-1 and NDST-2. Preparation of the cDNA clone to cover the complete open reading frame revealed that both NDST-3 and NDST-4 encode 872-873 amino acid residues (Aikawa and Esko 1999a,b) and exhibit a putative domain structure common with that of NDST-1 and NDST-2. It is composed of five domains as follows: cytoplasm, transmembrane, stem, N-deacetylase, and N-sulfotransferase domain in that order from the N-terminus (Berninsone and Hirschberg 1998; Sueyoshi et al. 1998). Enzyme activities for GlcNAc N-deacetylation and GlcN N-sulfation were detected in a recombinant form of human NDST-3 (Aikawa and Esko 1999a), and studies of those for NDST-4 are currently in progress (Aikawa and Esko 1999b). Because NDST-3 and NDST-4 can be prepared from both human and mouse, the presence of two new isozymes seems common in mammals.

Keywords

Heparan Sulfate Complete Open Reading Frame Phospho Sulfate Hexuronic Acid Fourth Member 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Jun-Ichi Aikawa
    • 1
  1. 1.Cellular Biochemistry LaboratoryRIKENSaitamaJapan

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