Chondroitin 4-Sulfotransferase

  • Osami Habuchi
  • Nobuyoshi Hiraoka


Chondroitin 4-sulfate, which contains a sulfate group on position 4 of GalNAc residues, is a major component of cartilage proteoglycan (aggrecan). Chondroitin 4- sulfate is also implicated in the celluar interaction with Plasmodium falciparum- infected erythrocytes (Rogerson et al. 1995; Fried and Duffy 1996). Chondroitin 4-sulfotransferase (C4ST) catalyzes the transfer of sulfate to position 4 of GalNAc residues of chondroitin and is involved in the biosynthesis of chondroitin 4-sulfate. cDNAs encoding mouse C4ST and human C4ST-1 and C4ST-2 have been cloned (Hiraoka et al. 2000; Yamauchi et al. 2000) mouse C4ST-1 is considered to be the mouse counterpart of human C4ST-1. All of the enzymes encoded by these cDNAs add 4-O- sulfate on dermatan sulfate using an in vitro assay. It remains to be studied whether C4ST is also involved in in vivo biosynthesis of dermatan sulfate.


Dermatan Sulfate Infected Erythrocyte Cartilage Proteoglycan Phospho Sulfate GalNAc Residue 
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  1. Albertini R, De Luca G, Passi A, Moratti R, Abuja PM (1999) Chondroitin-4-sulfate protects high-density lipoprotein against copper-dependent oxidation. Arch Biochem Biophys 365:143–149PubMedCrossRefGoogle Scholar
  2. Fried M, Duffy PE (1996) Adherence of Plasmodium falciparum to chondroitin sulfate A in the human placenta. Science 272:1416–1417CrossRefGoogle Scholar
  3. Habuchi O (2000) Diversity and functions of glycosaminoglycan sulfotransferases. Biochim Biophys Acta 1474:115–127PubMedCrossRefGoogle Scholar
  4. Habuchi O, Miyashita N (1982) Separation and characterization of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from chick embryo cartilage. Biochim Biophys Acta 717:414–421PubMedCrossRefGoogle Scholar
  5. Habuchi O, Miyata K (1980) Stimulation of glycosaminoglycan sulfotransferase from chick embryo cartilage by basic proteins and polyamines. Biochim Biophys Acta 616:208–217PubMedCrossRefGoogle Scholar
  6. Habuchi O, Matsui Y, Kotoya Y, Aoyama Y, Yasuda Y, Noda M (1993) Purfication of chondroitin 6-sulfotransferase secreted from cultured chick embryo chondrocytes. J Biol Chem 268:21968–21974PubMedGoogle Scholar
  7. Habuchi O, Tsuzuki M, Takeuchi I, Hara M, Matsui Y, Ashikari S (1991) Secretion of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase from cultured chick embryo chondrocytes. Biochim Biophys Acta 1133:9–16PubMedCrossRefGoogle Scholar
  8. Hiraoka N, Nakagawa H, Ong E, Akama OT, Fukuda MN, Fukuda M (2000) Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family. J Biol Chem 275:20188–20196PubMedCrossRefGoogle Scholar
  9. Kimata K, Okayama M, Oohira A, Suzuki S (1973) Cytodifferentiation and proteoglycan biosynthesis. Mol Cell Biochem 1:211–228PubMedCrossRefGoogle Scholar
  10. Naujokas MF, Morin M, Anderson MS, Peterson M, Miller J (1993) The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44. Cell 74:257–268PubMedCrossRefGoogle Scholar
  11. Robinson HC (1969) The sulphation of chondroitin sulphate in embryonic chicken cartilage. Biochem J 113:543–549PubMedGoogle Scholar
  12. Rogerson SJ, Chaiyaroj SC, Ng K, Reeder JC, Brown GV (1995) Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes. J Exp Med 182:15–20PubMedCrossRefGoogle Scholar
  13. Sugiura N, Sakurai K, Hori Y, Karasawa K, Suzuki S, Kimata K (1993) Preparation of lipid-derivatized glycosaminoglycans to probe a regulatory function of the carbohydrate moieties of proteoglycans in cell-matrix interaction. J Biol Chem 268:15779–15787PubMedGoogle Scholar
  14. Sugumaran G, Silbert JE (1988) Sulfation of chondroitin: specificity, degree of sulfation, and detergent effects with 4-sulfating and 6-sulfating microsomal systems. J Biol Chem 263:4673–4678PubMedGoogle Scholar
  15. Toyama-Sorimachi N, Sorimachi H, Tobita Y, Kitamura F, Yagita H, Suzuki K, Miyasaka M (1995) A novel ligand for CD44 is serglycin, a hematopoietic cell lineage-specific proteoglycan. Possible involvement in lymphoid cell adherence and activation. J Biol Chem 270:7437–7444PubMedCrossRefGoogle Scholar
  16. Yamagata M, Suzuki S, Akiyama SK, Yamada KM, Kimata K (1989) Regulation of cellsubstrate adhesion by proteoglycans immobilized on extracellular substrates. J Biol Chem 264:8012–8018PubMedGoogle Scholar
  17. Yamauchi S, Hirahara Y, Usui H, Takeda Y, Hoshino M, Fukuta M, Kimura JH, Habuchi O (1999) Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line. J Biol Chem 274:2456–2463PubMedCrossRefGoogle Scholar
  18. Yamauchi S, Mita S, Matsubara T, Fukuta M, Habuchi H, Kimata K, Habuchi O (2000) Molecular cloning and expression of chondroitin 4-sulfotransferase. J Biol Chem 275:8975–8981PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Osami Habuchi
    • 1
  • Nobuyoshi Hiraoka
    • 2
  1. 1.Department of Life ScienceAichi University of Education, Igaya, KariyaAichiJapan
  2. 2.Glycobiology ProgramCancer Research Center, The Burnham InstituteLa JollaUSA

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