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High Endothelial Cell N-Acetylglucosamine 6-O-Sulfotransferase

  • Annette Bistrup
  • Steven D. Rosen

Abstract

High endothelial cell (HEC)-specific N-acetylglucosamine 6-sulfotransferase (HEC- GlcNAc6ST) (Bistrup et al. 1999), or L-selectin ligand sulfotransferase (LSST) (Hiraoka et al. 1999), is a transmembrane protein of 386 amino acids with a short, N-terminal cytoplasmic tail and a large luminal C-terminal catalytic domain. This enzyme belongs to a recently identified family of carbohydrate sulfotransferases that modify the 6-hydroxyl of Gal, GalNAc, or GlcNAc residues (GST family) (Rosen et al. 1999; Hemmerich and Rosen 2000). In addition to HEC-GlcNAc6ST, there are two other GlcNAc-6 sulfotransferases in this family: N-acetylglucosamine 6- sulfotransferase [GlcNAc6ST (Uchimura et al. 1998a, b) or CHST2 (Li and Tedder 1999)] and intestinal N-acetylglucosamine 6-sulfotransferase (I-GlcNAc6ST) (Lee et al. 1999). Within their sulfotransferase domains, these three enzymes are more than 27% identical at the amino acid level and more than 56% similar. HEC-GlcNAc6ST is expressed in a limited number of tissues and is highly enriched in HECs (Bistrup et al. 1999; Hiraoka et al. 1999). In contrast, GlcNAc6ST is expressed ubiquitously (Uchimura et al. 1998b), and I-GlcNAc6ST is expressed almost exclusively in intestinal tissues (Lee et al. 1999). A recently identified chondroitin 6-O-sulfotransferase, designated C6ST-2 (Kitagawa et al. 2000), exhibits GalNAc 6-sulfotransferase activity toward chondroitin (Kitagawa et al. 2000). The other members of this novel family, keratan sulfate 6-sulfotransferase [KSGal6ST (Fukuta et al. 1997); CHST1 (Li and Tedder 1999)] and chondroitin 6-sulfotransferase (C6ST) (Fukuta et al. 1998), have galactose 6-sulfotransferase activity; in addition, C6ST catalyzes sulfation at C-6 of GalNAc in chondroitin sulfate (Fukuta et al. 1997).

Keywords

Keratan Sulfate Lymphocyte Homing Sialyl Lewisx High Endothelial Cell mRNA AF131235 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Annette Bistrup
    • 1
  • Steven D. Rosen
    • 1
  1. 1.Department of Anatomy and Program in ImmunologyUniversity of CaliforniaSan FranciscoUSA

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