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β3-Galactosyltransferase-V

  • Hisashi Narimatsu

Abstract

β3-Galactosyltransferase (β3GalT) transfers a galactose from UDP-Gal to N- acetylglucosamine (GlcNAc) with a β1,3-linkage. To date, five members of the β3GalT family have been cloned and analyzed (Issiki et al. 1999). β3GalT-I was first cloned by an expression cloning method (Sasaki et al. 1994). The other three members, β3GalT- II-IV, which have homologous sequences to β3GalT-I were found in the EST database and cloned (Issiki et al. 1999; Kolbinger et al. 1998; Amado et al. 1998; Hennet et al. 1998).

Keywords

Acceptor Substrate Namalwa Cell Genome Project Database Digestive Tract Tissue Galactosyltransferase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amado K, Almeida R, Carneiro F, Leverly SB, Holmes EH, Nomoto M, Hollingsworth MA, Hassan H, Schwientek T, Nielsen PA, Bennett EP, Clausen H (1998) A family of human β3-galactosyltransferases. Characterization of four members of a UDP-galactose: β-N-acetylglucosamine, β-N-acetylgalactosamine, β-1,3-galactosyltransferase family. J Biol Chem 278:12770–12778CrossRefGoogle Scholar
  2. Hennet T, Dinter A, Kuhnert P, Mattu TS, Rudd MP, Berger EG (1998) Genomic cloning and expression of three murine UDP-galactose:β-N-acetylglucosamine β1,3-galactosyltransferase genes. J Biol Chem 273:58–65PubMedCrossRefGoogle Scholar
  3. Holmes EH (1989) Preparative in vitro generation of lacto-series type-1 chain glycolipids catalyzed by β-1-3-galactosyltransferase from human colonic adenocarcinoma Colo 205 cells. Arch Biochem Biophys 270:630–646PubMedCrossRefGoogle Scholar
  4. Issiki S, Togayachi A, Kudo T, Nishihara S, Watanabe M, Kubota T, Kitajima M, Shiraishi N, Sasaki K, Andoh T, Narimatsu H (1999) Cloning, expression, and characterization of a novel UDP-galactoseβ-N-acetylglucosamine, β1,3-galactosyltransferase (β3GalT-V) responsible for synthesis of type-1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom. J Biol Chem 274:12499–12507CrossRefGoogle Scholar
  5. Kolbinger F, Streiff MB, Katopodis AG (1998) Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3β-galactosyltransferase catalyzing the formation of type-1 chains. J Biol Chem 273:433–440PubMedCrossRefGoogle Scholar
  6. Magnani JL, Steplewski Z, Koprowski H, Ginsburg V (1983) Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin. Cancer Res 43:5489–5492PubMedGoogle Scholar
  7. Nakayama T, Watanabe M, Katsumata T, Teramoto T, Kitajima M (1995) Expression of sialyl Lewis(a) as a new prognostic factor for patients with advanced colorectal carcinoma. Cancer 75:2051–2056PubMedCrossRefGoogle Scholar
  8. Narimatsu H, Iwasaki H, Nishihara S, Kimura H, Kudo T, Yamauchi Y, Hirohashi S (1996) Genetic evidence for the Lewis enzyme, which synthesizes type-1 Lewis antigens in colon tissue, and intracellular localization of the enzyme. Cancer Res 56:330–338PubMedGoogle Scholar
  9. Narimatsu H, Iwasaki H, Nakayama F, Ikehara Y, KudoT, Nishihara S, Sugano K, Okura H, Fujita S, Hirohashi S (1998) Lewis and secretor gene dosages affect CA19-9 and DUPAN-2 serum levels in normal individuals and colorectal cancer patients. Cancer Res 58:512–518PubMedGoogle Scholar
  10. Sasaki K, Sasaki E, Kawashima K, Hanai N, Nishi T, Hasegawa M (1994) JP0618759 A 940705Google Scholar
  11. Takada A, Ohmori K, Yoneda T, Tsuyuoka K, Hasegawa A, Kiso M, Kannagi R (1993) Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium. Cancer Res 53:354–361PubMedGoogle Scholar
  12. Valli M, Gallanti A, Bozzaro S, Trinchera M (1998) β-1,3-galactosyltransferase and α-1,2-fucosyltransferase involved in the biosynthesis of type-1-chain carbohydrate antigens in human colon adenocarcinoma cell lines. Eur J Biochem 256:494–501PubMedCrossRefGoogle Scholar
  13. Zhou D, Berger EG, Hennet T (1999) Molecular cloning of a human UDP-galactose: GlcNAcβ1,3GalNAcβ1,3-galactosyltransferase gene encoding an O-linked core-3-elongation enzyme. Eur J Biochem 263:571–576PubMedCrossRefGoogle Scholar
  14. Zhou D, Henion T, Jungalwala FB, Berger EG, Hennet T (2000) The β1,3-galactosyltrans-ferase β3GalT-V is a stage-specific embryonic antigen-3 (SSEA-3) synthase. J Biol Chem 275:22631–22634PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Hisashi Narimatsu
    • 1
  1. 1.Laboratory of Gene Function Analysis, Institute of Molecular and Cell Biology (IMCB)National Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

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