Advertisement

Keratan Sulfate Gal-6-Sulfotransferase

  • Masakazu Fukuta
  • Osami Habuchi

Abstract

Keratan sulfate (KS) is composed of the repeating disaccharide unit of Galβ1-4GlcNAc (poly-N-acetyllactosamine) with sulfate groups at the 6-position of the Gal and GlcNAc residues. Most GlcNAc residues and half of the Gal residues of corneal KS are sulfated, suggesting that sulfation of the Gal residue occurs after sulfation of the GlcNAc residue and determines the extent of sulfation of KS. Keratan sulfate Gal-6-sulfotransferase (KSGal6ST) catalyzes the transfer of sulfate to position 6 of the Gal residue of KS. Therefore it has been thought that KSGal6ST is responsible for producing the highly sulfated type of KS. KSGal6ST is also able to sulfate sialyl N-acetyllactosamine oligosaccharides. KSGal6ST may be engaged in the biosynthesis of not only KS but also glycoproteins bearing sulfated sialyl N-acetyllactosamine or sulfated sialyl Lewis X oligosaccharides.

Keywords

Synaptic Vesicle Keratan Sulfate Synaptic Vesicle Protein GlcNAc Residue Repeat Disaccharide Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bajjalieh SM, Peterson K, Shinghal R, Sheller RH (1992) SV2, a brain synaptic vesicle protein homologous to bacterial transporters. Science 257:1271–1273PubMedCrossRefGoogle Scholar
  2. Bistrup A, Bhakta S, Lee JK, Belov YY, Gunn MD, Zuo F-R, Huang C-C, Kannagi R, Rosen SD, Hemmerich S (1999) Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligand for L-selectin. J Cell Biol 145:899–910PubMedCrossRefGoogle Scholar
  3. Chakravarti S, Magnuson T, Lass JH, Jepsen KJ, LaMantia C, Carroll H (1998) Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican. J Cell Biol 141:1277–1286PubMedCrossRefGoogle Scholar
  4. Crowder KM, Gunther JM, Jones TA, Hale BD, Zhang HZ, Peterson MR, Sheller RH, Chavkin C, Bajjalieh SM (1999) Abnormal neurotransmission in mice lacking synaptic vesicle protein 2A. Proc Natl Acad Sci USA 96:15268–15273PubMedCrossRefGoogle Scholar
  5. Feany MB, Lee S, Edwards RH, Buckley KM (1992) The synaptic vesicle protein SV2 is a novel type of transmembrane transporter. Cell 70:861–867PubMedCrossRefGoogle Scholar
  6. Fukuta M, Inazawa J, Torii T, Tsuzuki K, Shimada E, Habuchi O (1997) Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase. J Biol Chem 272:32321–32328PubMedCrossRefGoogle Scholar
  7. Habuchi O (2000) Diversity and functions of glycosaminoglycan sulfotransferases. Biochim Biophys Acta 1474:115–127PubMedCrossRefGoogle Scholar
  8. Habuchi O, Matsui Y, Kotoya Y, Aoyama Y, Yasuda Y, Noda M (1993) Purification of chondroitin 6-sulfotransferase secreted from cultured chick embryo chondrocytes. J Biol Chem 268:21968–21974PubMedGoogle Scholar
  9. Hasegawa N, Torii T, Kato T, Miyajima H, Nakayasu K, Kanai A, Habuchi O (1999) Determination of activities of galactose 6-O-sulfotransferase and N-acetylglucosamine 6-O-sulfotransferase in the cornea of patients with macular corneal dystrophy. Glycoconj J 16:S155Google Scholar
  10. Janz R, Goda Y, Geppert M, Missler M, Südhof TC (1999) SV2A and SV2B function as redundant Ca2+ regulators in neurotransmitter release. Neuron 24:1003–1016PubMedCrossRefGoogle Scholar
  11. Lindahl B, Erickson L, Spillmann D, Caterson B, Lindahl U (1996) Selective loss of cerebral keratan sulfate in Alzheimer’s disease. J Biol Chem 271:16991–16994PubMedCrossRefGoogle Scholar
  12. Midura RJ, Hascall VC, MacCallum DK, Meyer RF, Thonar EJ-MA, Hassell JR, Smith CF, Klintworth GK (1990) Proteoglycan biosynthesis by human corneas from patients with type 1 and 2 macular corneal dystrophy. J Biol Chem 265:15947–15955PubMedGoogle Scholar
  13. Nakazawa K, Hassel JR, Hascall VC, Lohmander S, Newsome DA, Krachmer J (1984) Defective processing of keratan sulfate in macular corneal dystrophy. J Biol Chem 259:13751–13757PubMedGoogle Scholar
  14. Rahamimoff R, Fernandez JM (1997) Pre-and postfusion regulation of transmitter release. Neuron 18:17–27PubMedCrossRefGoogle Scholar
  15. Rütter ER, Kresse H (1984) Partial purification and characterization of 3′-phosphoadeny-lylsulfate:keratan sulfate sulfotransferases. J Biol Chem 259:11771–11776Google Scholar
  16. Scranton TW, Iwata M, Carlson SS (1993) The SV2 protein of synaptic vesicles is a keratan sulfate proteoglycan. J Neurochem 61:29–44PubMedCrossRefGoogle Scholar
  17. Torii T, Fukuta M, Habuchi O (2000) Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase. Glycobiology 10:203–211PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Masakazu Fukuta
    • 1
  • Osami Habuchi
    • 1
  1. 1.Department of Life ScienceAichi University of Education, Igaya-cho, KariyaAichiJapan

Personalised recommendations