ST8Sia-V was the fifth molecule cloned as an α2-8-sialyltransferase. This enzyme exists as three isoforms as ST8Sia-V-L, -M, -S in mice. The predicted amino acid sequences of mouse ST8Sia-V-L, -M, -S are 412, 376, and 345, respectively, all of which exhibit a type II transmembrane topology, consisting of an NH2 terminal cytoplasmic tail, a transmembrane domain, a proline-rich stem region, and a large COOH- terminal active domain. The nucleotide sequences of the three isoforms are identical to each other except in the putative stem region (Kono et al. 1996).
KeywordsTransmembrane Topology Ganglioside Biosynthesis Acceptor Substrate Specificity Mouse Brain cDNA Library Oligonucleotide Prime Base
Unable to display preview. Download preview PDF.