• Naoya Kojima
  • Shuichi Tsuji


ST8Sia-II (STX) belongs to a family of α2-8-sialyltransferases, and exhibits polysialic acid synthase activity like ST8Sia-IV (see Chap. 48). The cDNAs encoding ST8Sia-II have been cloned from rat (Livingston and Paulson 1993), mouse (Kojima et al. 1995a), human (Scheidegger et al. 1995; Angata et al. 1997), and Xenopus (Kudo et al. 1998). The deduced amino acid sequences of the ST8Sia-II cloned from human, mouse, and rat exhibit the same 375 amino acid lengths, and conserve over 99% among them. The amino acid sequence of ST8Sia-II cloned from mouse showed 34.4% and 56.0% identity with mouse ST8Sia-III and mouse ST8Sia-IV, respectively, but no similarity of the amino acid sequence (less than 30%) of mouse ST8Sia-II was observed toward those of other sialyltransferases cloned from mouse. Like other sialyltransferases, ST8Sia- II exhibits type II membrane protein topology and has characteristic motifs for sialvltransferases called sialvlmotifs L, S, and VS.


Sialic Acid Deduce Amino Acid Sequence Neural Cell Adhesion Molecule Neuro2a Cell Sialic Acid Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Angata K, Nakayama J, Fredette B, Chong K, Ranscht B, Fukuda M (1997) Human STX polysialyltransferase forms the embryonic form of the neural cell adhesion molecule. Tissue-specific expression, neurite outgrowth, and chromosomal localization in comparison with another polysialyltransferase, PST. J Biol Chem 272:7182–7190PubMedCrossRefGoogle Scholar
  2. Angata K, Suzuki M, Fukuda M (1998) Differential and cooperative polysialylation of the neural cell adhesion molecule by two polysialyltransferases, PST and STX. J Biol Chem 273:28524–28532PubMedCrossRefGoogle Scholar
  3. Eckhardt M, Muhlenhoff M, Bethe A, Koopman J, Frosch M, Gerardy-Schahn R (1995) Molecular characterization of eukaryotic polysialyltransferase-1. Nature 373:715–718PubMedCrossRefGoogle Scholar
  4. Kiss JZ, Rougon G (1997) Cell biology of polysialic acid. Curr Opin Neurobiol 7:640–646PubMedCrossRefGoogle Scholar
  5. Kojima N, Yoshida Y, Kurosawa N, Lee YC, Tsuji S (1995a) Enzymatic activity of a devel-opmentally regulated member of the sialyltransferase family (STX): evidence for α2,8-sialyltransferase activity toward N-linked oligosaccharides. FEBS Lett 360:1–4PubMedCrossRefGoogle Scholar
  6. Kojima N, Yoshida Y, Tsuji S (1995b) A developmentally regulated member of the sialyltransferase family (ST8Sia-II, STX) is a polysialic acid synthase. FEBS Lett 373:119–122PubMedCrossRefGoogle Scholar
  7. Kojima N, Tachida Y, Yoshida Y, Tsuji S (1996) Characterization of mouse ST8Sia-II (STX) as a neural cell adhesion molecule-specific polysialic acid synthase. Requirement of core α1,6-linked fucose and a polypeptide chain for polysialylation. J Biol Chem 271:19457–19463PubMedCrossRefGoogle Scholar
  8. Kojima N, Tachida Y, Tsuji S (1997) Two polysialic acid synthases, mouse ST8Sia-II and-IV, synthesize different degrees of polysialic acids on different substrate glycoproteins in mouse neuroblastoma Neuro2a cells. J Biochem (Tokyo) 122:1265–1273CrossRefGoogle Scholar
  9. Kudo M, Takayama E, Tashiro K, Fukamachi H, Nakata T, Tadakuma T, Kitajima K, Inoue Y, Shiokawa K (1998) Cloning and expression of an α-2,8-polysialyltransferase (STX) from Xenopus laevis. Glycobiology 8:771–777PubMedCrossRefGoogle Scholar
  10. Kurosawa N, Yoshida Y, Kojima N, Tsuji S (1997) Polysialic acid synthase (ST8Sia-II/STX) mRNA expression in the developing mouse central nervous system. J Neurochem 69:494–503PubMedCrossRefGoogle Scholar
  11. Livingston BD, Paulson JC (1993) Poly mer ase chain reaction cloning of a developmentally regulated member of the sialyltransferase gene family. J Biol Chem 268:11504–11507PubMedGoogle Scholar
  12. Nakayama J, Fukuda M (1996) A human polysialyltransferase directs in vitro synthesis of polysialic acid. J Biol Chem 271:1829–1832PubMedCrossRefGoogle Scholar
  13. Rutishauser U (1998) Polysialic acid at the cell surface: biophysics in service of cell interactions and tissue plasticity. J Cell Biochem 70:304–312PubMedCrossRefGoogle Scholar
  14. Rutishauser U, Landmesser L (1996) Polysialic acid in the vertebrate nervous system: a promoter of plasticity in cell-cell interactions. Trends Neurosci 19:422–427PubMedGoogle Scholar
  15. Scheidegger EP, Sternberg LR, Roth J, Lowe JB (1995) A human STX cDNA confers polysialic acid expression in mammalian cells. J Biol Chem 270:22685–22688PubMedCrossRefGoogle Scholar
  16. Takashima S, Yoshida Y, Kanematsu T, Kojima N, Tsuji S (1998) Genomic structure and promoter activity of the mouse polysialic acid synthase (mST8Sia-IV/PST) gene. J Biol Chem 273:7675–7683PubMedCrossRefGoogle Scholar
  17. Tsuji S, Datta AK, Paulson JC (1996) Systematic nomenclature for sialyltransferases. Glycobiology 6(7):v–viiPubMedCrossRefGoogle Scholar
  18. Yoshida Y, Kurosawa N, Kanematsu T, Kojima N, Tsuji S (1996) Genomic structure and promoter activity of the mouse polysialic acid synthase gene (mST8Sia-II). Brain-specific expression from a TATA-less GC-rich sequence. J Biol Chem 271:30167–30173PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Naoya Kojima
    • 1
  • Shuichi Tsuji
    • 2
  1. 1.Department of Applied ChemistryTokai UniversityKanagawaJapan
  2. 2.Department of Chemistry, Faculty of ScienceOchanomizu University, Otsuka, Bunkyo-kuTokyoJapan

Personalised recommendations