ST6GalNAc-II is a CMP-sialic acid: N-acetylgalactosaminide α2-6-sialyltransferase (GalNAc α2-6-sialyltransferase), and the second member of the ST6GalNAc subfamily that exhibits activity toward GalNAc-O-Ser/Thr, Galβ1,3GalNAc-O-Ser/Thr, and NeuAcα2,3Galβ1,3GalNAc-O-Ser/Thr (Kurosawa et al. 1994, 1996; Kono et al. 2000). Like other sialyltransferases, ST6GalNAc-II exhibits type II membrane protein topology and has characteristic motifs for sialyltransferases called sialylmotifs L, S, and VS. ST6GalNAc-II also has a Kurosawa motif (Cys-Xaa75–82-Cys-Xaa-Cys-Ala-Xaa- Vα1-Xaa150-in160-Cys; Xaa denotes any amino acid residue) as seen in the ST3Gal family and two members of the ST6GalNAc family (Kurosawa et al. 1996; Tsuji 1999). Mouse ST6GalNAc-II cDNA encodes a protein of 373 amino acids, showing low identity to chick ST6GalNAc-II (57%) and -I (38%), and mouse ST6GalNAc-I (41%), -III (20%), -IV (22%), and -V (20%).
KeywordsMammary Gland Acceptor Substrate Fusion Enzyme Amino Acid Codon Similar Substrate Specificity
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