Abstract
GDP-L-FucN-acetyl-β-D-glucosaminide α1-6fucosyltransferase (FUT8) catalyzes the transfer of fucose from GDP-Fuc to N-linked-type complex glycoproteins, as shown in Fig. 1. The enzymatic products, α1,6-fucosylated (core fucosylated) N-glycans, are commonly observed in many glycoproteins, and are especially abundant in brain tissue. It is well known that the sugar chains in α-fetoprotein (AFP), a well-known tumor marker of hepatocellular carcinoma, are microheterogenous in nature to sugar chains. The oligosaccharide structures of transferrin as well as AFP, synthesized by hepatocellular carcinoma cells, are highly fucosylated (Champion et al. 1989). In contrast, FUT8 is released from platelets during blood clotting (Koscielak et al. 1987), suggesting that this enzyme might play a role in blood coagulation. An increase in fucosylated carbohydrates in pathological conditions has also been reported in other types of cancer cells (Tatsumura 1977).
Reaction pathway of FUT8. GlcNAc indicates N-acetylglucosamine, Man indicates mannose, Fuc indicates fucose, GDP-Fuc indicates guanosinediphosphofucopyranoside, and Asn indicates asparagine
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References
Champion B, Leger D, Wieruszeski J-M, Montreuil J, Spik G (1989) Presence of fucosylated triantennary, tetraantennary and pentaantenary glycans in transferrin synthesized by the human hepatocarcinoma cell line HepG2. Eur J Biochem 184:405–413
Kaminska J, Glick MC, Koscielak J (1998) Purification and characterization of GDP-1-FucN-acetyl β-D-glucosaminide α1→6-fucosyltransferase from human blood platelets. Glycoconj J 15:783–788
Koscielak J, Pacuszka T, Kubin J, Zdziechowska H (1987) Serum α-6-L-fucosyltransferase is released from platelets during clotting of blood. Glycoconj J 4:43–49
Miyoshi E, Noda K, Ko JH, Ekuni A, Kitada T, Uozumi N, Ikeda Y, Matsuura N, Sasaki Y, Hayashi N, Hori M, Taniguchi N (1999) Overexpression of α1-6 fucosyltransferase in hepatoma cells suppresses intrahepatic metastasis after splenic injection in athymic mice. Cancer Res 59:2237–2243
Noda K, Miyoshi E, Uozumi N, Ikeda Y, Gao C-X, Suzuki K, Yoshihara H, Yoshikawa K, Kawano K, Hayashi N, Hori M, Taniguchi N (1998) Gene expression of α1-6 fucosyltransferase in human hepatoma tissues: a possible implication for increased fucosylation of α fetoprotein. Hepatology 28:944–952
Scanlin TF, Wang YM, Glick MC (1985) Altered fucosylation of membrane glycoproteins from cystic fibrosis fibroblasts. Pediatr Res 19:368–374
Tatsumura T, Sato H, Mori A, Komori Y, Yamamoto K, Fukutani G, Kuno S (1977) Clinical significance of fucose level in glycoprotein fraction of serum in patients with malignant tumors. Cancer Res 37:4101–4103
Uozumi N, Teshima T, Yamamoto T, Nishikawa A, Gao YE, Miyoshi E, Gao CX, Noda K, Islam KN, Ihara Y, Fujii S, Shiba T, Taniguchi N (1996a) A fluorescent assay method for GDP-L-FucN-acetyl-β-D-glucosaminide α1-6fucosyltransferase activity, involving high-performance liquid chromatography. J Biochem 120:385–392
Uozumi N, Yanagidani S, Miyoshi E, Ihara Y, Sakuma T, Gao CX, Teshima T, Fujii S, Shiba T, Taniguchi N (1996b) Purification and cDNA cloning of porcine brain GDP-1-FUC:N-acetyl-β-D-glucosaminide:α1-6-fucosyltransferase. J Biol Chem 271:27810–27817
Voynow JA, Kaiser RS, Scanlin TF, Glick MC (1991) Purification and characterization of GDP-1-FUC: N-acetyl-β-D-glucosaminide α1-6-fucosyltransferase from cultured human skin fibroblasts. J Biol Chem 266:21572–21577
Wang YM, Hare TR, Won B, Stowell CP, Scanlin TF, Glick MC, Hard K, van Kuik JA, Vliegen-thart JF (1990) Additional fucosyl residues on membrane glycoproteins but not a secreted glycoprotein from cystic fibrosis fibroblasts. Clin Chim Acta 188:193–210
Wilson JR, Willams D, Schachter H (1976) The control of glycoprotein synthesis: N-acetyl-glucosamine linkage to α-mannose residue as a signal of L-fucose to the asparagine-linked N-acetylglucosamine residue of glycopeptide from α1-acid glycoprotein. Biochem Biophys Res Commun 72:909–916
Yamaguchi Y, Fujii J, Inoue S, Uozumi N, Yanogidani S, Ikeda Y, Egashira M, Miyoshi O, Niikawa N, Taniguchi N (1999) Mapping of the od,6-fucosyltransferase gene, FUT8, to human chromosome 14q24.3. Cytogenet. Cell Genet 84, 58–60
Yanagidani S, Uozumi N, Ihara Y, Miyoshi E, Yamaguchi N, Taniguchi N (1997) Purification and cDNA cloning of GDP-L-Fuc:N-acetylglucosaminide:α1-6 fucosyltransferase (FUT8) from human stomach carcinoma MKN45 cells. J Biochem 121:626–632
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Miyoshi, E., Taniguchi, N. (2002). α6-Fucosyltransferase (FUT8). In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_34
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DOI: https://doi.org/10.1007/978-4-431-67877-9_34
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