α1,3-Fucosyltransferase-VII (Fuc-TVII, FUT7) was cloned independently by two groups at almost the same time (Sasaki et al. 1994; Natsuka et al. 1994). Sasaki et al. first cloned the FUT7 cDNA from a THP-1 cell cDNA library by an expression cloning method using an anti-sLex mAb KM93. Natsuka et al. employed a cross-hybridization method for cloning the FUT7 cDNA. Flow cytometric analysis on the cells stably expressing FUT7 demonstrated that FUT7 can form sLex but not Lex on the cell surface. FUT7 can transfer fucose only to the GlcNAc residue of the sialylated type-2 chain but not to that of type-1 chain. Thus, FUT7 can synthesize only the sLex epitope and not the other Lewis antigens such as Lex, Lea, Leb, and sLea. The tissue distribution of FUT7 is limited to leukocytes and high-endothelial venules (HEV) (Maly et al. 1996; Clarke and Watkins 1996; Marer et al. 1997; Kaneko et al. 1999).
KeywordsLewis Antigen Selectin Ligand Sialyl Lewisx Polylactosamine Chain sLeX Epitope
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