Advertisement

α3-Fucosyltransferase-VII (FUT7)

  • Hisashi Narimatsu

Abstract

α1,3-Fucosyltransferase-VII (Fuc-TVII, FUT7) was cloned independently by two groups at almost the same time (Sasaki et al. 1994; Natsuka et al. 1994). Sasaki et al. first cloned the FUT7 cDNA from a THP-1 cell cDNA library by an expression cloning method using an anti-sLex mAb KM93. Natsuka et al. employed a cross-hybridization method for cloning the FUT7 cDNA. Flow cytometric analysis on the cells stably expressing FUT7 demonstrated that FUT7 can form sLex but not Lex on the cell surface. FUT7 can transfer fucose only to the GlcNAc residue of the sialylated type-2 chain but not to that of type-1 chain. Thus, FUT7 can synthesize only the sLex epitope and not the other Lewis antigens such as Lex, Lea, Leb, and sLea. The tissue distribution of FUT7 is limited to leukocytes and high-endothelial venules (HEV) (Maly et al. 1996; Clarke and Watkins 1996; Marer et al. 1997; Kaneko et al. 1999).

Keywords

Lewis Antigen Selectin Ligand Sialyl Lewisx Polylactosamine Chain sLeX Epitope 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bengtson P, Larson C, Lundblad A, Larson G, Pahlsson P (2001) Identification of a missense mutation (G329A; Arg110-Gln) in the human FUT7 gene. J Biol Chem 276:31575–31582PubMedCrossRefGoogle Scholar
  2. Clarke JL, Watkins WM (1996) α1,3-L-Fucosyltransferase expression in developing human myeloid cells. J Biol Chem 271:10317–10328PubMedCrossRefGoogle Scholar
  3. Fuhlbrigge RC, Kieffer JD, Armerding D, Kupper TS (1997) Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389:978–981PubMedCrossRefGoogle Scholar
  4. Grabenhorst E, Nimtz M, Costa J, Conradt HS (1998) In vivo specificity of human α13/l,4-fucosyltransferases III-VII in the biosynthesis of Lewisx and sialyl Lewisx motifs on complex-type N-glycans. J Biol Chem 273:30985–30994PubMedCrossRefGoogle Scholar
  5. Hiraiwa N, Dohi T, Kawakami-Kimura N, Yumen M, Ohmori K, Maeda M, Kannagi R (1996) Suppression of sialyl Lewis X expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection of antisense cDNA of an α1,3fucosyltransferase (Fuc-TVII). J Biol Chem 271:31556–31561PubMedCrossRefGoogle Scholar
  6. Hiraiwa N, Hiraiwa M, Kannagi R (1997) Human T-cell leukemia virus-1 encoded Tax protein transactivates α1,3-fucosyltransferase Fuc-TVII, which synthesizes sialyl Lewis X, a selectin ligand expressed on adult T-cell leukemia cells. Biochem Biophys Res Commun 231:183–186PubMedCrossRefGoogle Scholar
  7. Huang M-C, Zollner O, Moll T, Maly P, Thall AD, Lowe JB, Vestweber D (2000) P-selectin glycoprotein ligand-1 and E-selectin ligand-1 are differentially modified by fucosyltransferases Fuc-TIV and Fuc-TVII in mouse neutrophils. J Biol Chem in pressGoogle Scholar
  8. Kaneko M, Kudo T, Iwasaki H, Ikehara Y, Nishihara S, Nakagawa S, Sasaki K, Shiina T, Inoko H, Saitou N, Narimatsu H (1999) α1,3-fucosyltransferase IX (Fuc-TIX) is very highly conserved between human and mouse; molecular cloning, characterization and tissue distribution of human Fuc-TIX. FEBS Lett 452:237–242PubMedCrossRefGoogle Scholar
  9. Kimura H, Shinya N, Nishihara S, Kaneko M, Irimura T, Narimatsu H (1997) Distinct substrate specificities of five human α-1,3-fucosyltransferases for in vivo synthesis of the sialyl Lewis X and Lewis X epitopes. Biochem Biophys Res Commun 237:131–137PubMedCrossRefGoogle Scholar
  10. Knibbs RN, Craig AC, Natsuka S, Chang A, Cameron M, Lowe JB, Stoolman LM (1996) The fucosyltransferase Fuc-TVII regulates E-selectin ligand synthesis in human T cells. J Cell Biol 133:911–920PubMedCrossRefGoogle Scholar
  11. Maly P, Thall AD, Petryniak B, Rogers CE, Smith PL, Marks RM, Kelly RJ, Gersten KM, Cheng G, Saunders TL, Camper SA, Camphausen RT, Sullivan FX, Isogai Y, Hindsgall O, von Andrian UH, Lowe JB (1996) The α(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis. Cell 86:643–653PubMedCrossRefGoogle Scholar
  12. Marer NL, Palcic MM, Clarke JL, Davies D, Skacel PO (1997) Developmental regulation of α13-fucosyltransferase expression in CD34 positive progenitors and maturing myeloid cells isolated from normal bone marrow. Glycobiology 7:357–365PubMedCrossRefGoogle Scholar
  13. Mitsuoka C, Sawada-Kasugai M, Ando-Fukui K, Nakanishi H, Nakamura S, Ishida H, Kiso M, Kannagi R (1998) Identification of a major carbohydrate capping group of the L-selectin ligand on high-endothelial venules in human lymph nodes as 6-sulfo sialyl Lewis X. J Biol Chem 273:11225–11233PubMedCrossRefGoogle Scholar
  14. Nakamura M, Kudo T, Narimatsu H, Furukawa Y, Kikuchi J, Asakura S, Yang W, Iwase S, Hatake K, Miura Y (1998) Single glycosyltransferase, Core 2 β1-6-N-acetylglucosaminyltransferase, regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester. J Biol Chem 273:26779–26789PubMedCrossRefGoogle Scholar
  15. Nakayama F, Teraki Y, Kudo T, Togayachi A, Iwasaki H, Tamatani T, Nishihara S, Mizukawa Y, Shiohara T, Narimatsu H (2000) Expression of cutaneous lymphocyte-associated antigen regulated by a set of glycosyltransferases in human T cells: involvement of α1,3-fucosyltransferase VII and β1,4-galactosyltransferase I. J Invest Dermatol 115:299–306PubMedCrossRefGoogle Scholar
  16. Natsuka S, Gersten KM, Zenita K, Kannagi R, Lowe JB (1994) Molecular cloning of a cDNA encoding a novel human leukocyte alphα-1,3-fucosyltransferase capable of synthesizing the sialyl Lewis X determinant. J Biol Chem 269:16789–16794PubMedGoogle Scholar
  17. Niemela R, Natunen J, Majuri M-L, Maaheimo H, Helin J, Lowe JB, Renkonen O, Renkonen R (1998) Complementary acceptor and site specificity of Fuc-TIV and Fuc-TVII allow effective biosynthesis of sialyl-triLex and related polylactosamines present on glycoprotein counterreceptors of selectins. J Biol Chem 273:4021–4026PubMedCrossRefGoogle Scholar
  18. Sasaki K, Kurata K, Funayama K, Nagata M, Watanabe E, Ohta S, Hanai N, Nishi T (1994) Expression cloning of a novel alpha 1,3-fucosyltransferase that is involved in biosynthesis of the sialyl Lewis X carbohydrate determinants in leukocytes. J Biol Chem 269:14730–14737PubMedGoogle Scholar
  19. Shinoda K, Morishita Y, Sasaki K, Matsuda Y, Takahashi I, Nishi T (1997) Enzymatic characterization of human α1,3-fucosyltransferase Fuc-TVII synthesized in a B cell lymphoma cell line. J Biol Chem 272:31992–31997PubMedCrossRefGoogle Scholar
  20. Shinoda K, Tanahashi E, Fukunaga K, Ishida H, Kiso M (1998) Detailed acceptor specificities of human α-1,3-fucosyltransferases, Fuc-TVII and Fuc-TVI. Glycoconj J 15:969–974PubMedCrossRefGoogle Scholar
  21. Smith PL, Gersten KM, Bronislawa P, Kelly RJ, Rogers C, Natsuka Y, Alford III JA, Scheidegger EP, Natsuka S, Lowe JB (1996) Expression of the α(1,3)fucosyltransferase Fuc-TVII in lymphoid aggregate high-endothelial venules correlates with expression of L-selectin ligands. J Biol Chem 271:8250–8259PubMedCrossRefGoogle Scholar
  22. Wagers A, Lowe JB, Kansas GS (1996) An important role for the α1,3fucosyltransferase, Fuc-TVII, in leukocyte adhesion to E-selectin. Blood 88:2125–2132PubMedGoogle Scholar
  23. Wagers AJ, Waters CM, Stoolman LM, Kansas GS (1998) Interleukin 12 and interleukin 4 control T cell adhesion to endothelial selectins through opposite effects on α1,3-fucosyltransferase VII gene expression. J Exp Med 188:2225–2231PubMedCrossRefGoogle Scholar
  24. Weninger W, Ulfman LH, Cheng G, Souchkova N, Quackenbush EJ, Lowe JB, von Andrian UH (2000) Specialized contributions by α(1,3)-fucosyltransferase-IV and Fuc-T-VII during leukocyte rolling in dermal microvessels. Immunity 12:665–676PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Hisashi Narimatsu
    • 1
  1. 1.Laboratory of Gene Function Analysis, Institute of Molecular and Cell Biology (IMCB)National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan

Personalised recommendations