α2-Fucosyltransferases (FUT1,FUT2, and Sec1)

  • Rafael Oriol
  • Rosella Mollicone


The human FUT1 and FUT2 α2-fucosyltransferases corresponding to the H and Se enzymes transfer Fuc in α1,2-linkages onto the terminal galactose of lactosamine. Individuals of histoblood-group “O” express the H antigen under control of the FUT1 gene on red cells and vascular endothelium, and the H antigen under control of the FUT2 gene in exocrine secretions.


Blood Group Exocrine Secretion Acceptor Substrate High Evolutionary Rate Terminal Galactose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Apoil PA, Roubinet F, Despiau S, Mollicone R, Oriol R, Blancher A (2000) Evolution of α2-fucosyltransferase genes in primates: relation between intronic Alu-Y element and red cell expression of ABH antigens. Mol Biol Evol 17:337–351PubMedCrossRefGoogle Scholar
  2. Barreaud JP, Saunier K, Souchaire J, Delourme D, Oulmouden A, Oriol R, Leveziel H, Julien R, Petit FM (2000) Three bovine α2-fucosyltransferase genes encode enzymes that preferentially transfer fucose on Galβ1, 3GalNAc acceptor substrates. Glycobiology 10:611–621PubMedCrossRefGoogle Scholar
  3. Bhende YM, Deshpande CK, Bhatia HM, Sänger R, Race RR, Morgan WTJ, Watkins WM (1952) A new blood group character related to the ABO system. Lancet i:903–904Google Scholar
  4. Breton C, Oriol R, Imberty A (1996) Sequence alignment and fold recognition of fucosyltransferases. Glycobiology 6:vii–xiiPubMedCrossRefGoogle Scholar
  5. Breton C, Oriol R, Imberty A (1998) Conserved structural features in eukaryotic and prokaryotic fucosyltransferases. Glycobiology 8:87–94PubMedCrossRefGoogle Scholar
  6. Costache M, Apoil P, Cailleau A, Elmgren A, Larson G, Henry S, Blancher A, Iordachescu D, Oriol R, Mollicone R (1997) Evolution of fucosyltransferase genes in vertebrates. J Biol Chem 272:29721–29728PubMedCrossRefGoogle Scholar
  7. Decastello AV, Sturli A (1902) Uber die isoagglutinine im serum gesunder und kranker menschen. Munchen Med Wschr 11:1090–1095Google Scholar
  8. Faik A, Bar-Peled M, DeRocher AE, Zeng W, Perrin RM, Wilkersson C, Raikhel NV, Keegstra K (2000) Biochemical characterization and molecular cloning of an α-1,2-fucosyltransferase that catalyzes the last step of cell wall xyloglucan biosynthesis in pea. J Biol Chem 275:15082–15089PubMedCrossRefGoogle Scholar
  9. Fernandez-Mateos P, Cailleau A, Henry S, Costache M, Elmgren A, Svenson L, Larson G, Samuelsson BE, Oriol R, Mollicone R (1998) Point mutations and deletion responsible for the Bombay H null and the Reunion H weak blood groups. Vox Sang 75:37–46PubMedCrossRefGoogle Scholar
  10. Gastinel LN, Cambillau C, Bourne Y (1999) Crystal structures of the bovine β4-galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose. EMBO J 18:3546–3557PubMedCrossRefGoogle Scholar
  11. Henry S, Mollicone R, Fernandez P, Samuelsson B, Oriol R, Larson G (1996a) Homozygous expression of a missense mutation at nucleotide 385 in the FUT2 gene associates with the Le(a+b+) partiα1-secretor phenotype in an Indonesian family. Biochem Biophys Res Com 219:675–678PubMedCrossRefGoogle Scholar
  12. Henry S, Mollicone R, Fernandez P, Samuelsson B, Oriol R, Larson G (1996b) Molecular basis for erythrocyte Le(a+b+) and salivary ABH partiα1-secretor phenotypes. Expression of a FUT2 secretor allele with an A-T point mutation at nucleotide 385 correlates with reduced α(1,2)fucosyltransferase activity. Glycoconj J 13:985–993PubMedCrossRefGoogle Scholar
  13. Henry S, Mollicone R, Lowe J, Samuelsson BE, Larson GA (1996c) A second nonse-cretor allele of the blood group α(1,2)fucosyltransferase gene (FUT2). Vox Sang 70:1–25CrossRefGoogle Scholar
  14. Johnson PH, Mak MK, Leong S, Broadberry R, Duraisamy G, Gooch A, Lin CM, Makar I, Okubo Y, Swart E, Kolpsall E, Ewess M (1994) Analysis of mutations in the blood group H gene in donors with H-deficient phenotypes. Vox Sang 67:Suppl 2, 25 (abstract)CrossRefGoogle Scholar
  15. Kaneko M, Nishihara S, Shinya N, Kudo T, Iwasaki H, Seno T, Okubo Y, Narimatsu H (1997) Wide variety of point mutations in the H gene of Bombay and para-Bombay individuals that inactivate H enzyme. Blood 90:839–849PubMedGoogle Scholar
  16. Kelly RJ, Ernst LK, Larsen RD, Bryant JG, Robinson JS, Lowe JB (1994) Molecular basis for H blood group deficiency in Bombay (Oh) and para-Bombay individuals. Proc Natl Acad Sci USA 91:5843–5847PubMedCrossRefGoogle Scholar
  17. Kelly RJ, Rouquier S, Giorgi D, Lennon GG, Lowe JB (1995) Sequence and expression of a candidate for the human secretor blood group α(1,2)fucosyltransferase gene (FUT2). J Biol Chem 270:4640–4649PubMedCrossRefGoogle Scholar
  18. Koda Y, Soejima M, Liu Y, Kimura H (1996) Molecular basis for secretor type α(1,2)fucosyltransferase gene deficiency in the Japanese population: a fusion gene generated by unequal crossover responsible for a nonsecretor phenotype. Am J Hum Genet 59:343–3PubMedGoogle Scholar
  19. Koda Y, Soejima M, Johnson PH, Smart E, Kimura H (1997) Missense mutation of FUT1 and deletion of FUT2 are responsible for the Indian Bombay phenotype of the ABO blood group system. Biochem Biophys Res Commun 238:21–25PubMedCrossRefGoogle Scholar
  20. Koda Y, Soejima M, Johnson P, Smart E, Kimura H (2000a) An Alu-mediated large deletion of the FUT2 gene in individuals with the ABO-Bombay phenotype. Hum Genet 106:80–PubMedCrossRefGoogle Scholar
  21. Koda Y, Tachida H, Soejima M, Takenaka O, Kimura H (2000b) Ancient origin of the null allele se428 of the human ABO-secretor locus (FUT2). J Mol Evol 50:243–248PubMedGoogle Scholar
  22. Kudo T, Iwasaki H, Nishihara S, Shinya N, Ando T, Narimatsu I, Narimatsu H (1996) Molecular genetic analysis of the human Lewis histoblood group system. II. Secretor gene inactivation by a novel single missense mutation A385-T in Japanese nonsecretor individuals. J Biol Chem 271:9830–9837PubMedCrossRefGoogle Scholar
  23. Kumazaki T, Yoshida A (1984) Biochemical evidence that the secretor gene, Se, is a structural gene encoding a specific fucosyltransferase. Proc Natl Acad Sci USA 81:4193–4197PubMedCrossRefGoogle Scholar
  24. Landsteiner K (1901) Uber agglutinationserscheinungen normalen menschlichen blutes. Klin Wochenschr 14:1132–1134Google Scholar
  25. Larsen RD, Ernst LK, Nair RP, Lowe JB (1990) Molecular cloning, sequence and expression of a human GDP-L-fucose: β-D-galactoside α2-L-fucosyltransferase cDNA that can form the H blood group antigen. Proc Natl Acad Sci USA 87:6674–6678PubMedCrossRefGoogle Scholar
  26. Le Pendu J, Cartron JP, Lemieux RU, Oriol R (1985) The presence of at least two different H-blood group related β-D-Gal α-2-L-fucosyltransferases in human serum and the genetics of blood group H substances. Am J Hum Genet 37:749–760PubMedGoogle Scholar
  27. Levine P, Robinson E, Celano M, Briggs O, Falkinburg L (1955) Gene interactions resulting in suppression of blood group substance B. Blood 10:1100–1108PubMedGoogle Scholar
  28. Liu YH, Koda Y, Soejima M, Pang H, Schlaphoff T, duToit ED, Kimura H (1998) Extensive polymorphism of the FUT2 gene in an African (Xhosa) population of South Africa. Hum Genet 103:204–210PubMedCrossRefGoogle Scholar
  29. Liu YH, Koda Y, Soejima M, Pang H, Wang BJ, Kim DS, Oh HB, Kimura H (1999) The fusion gene at the ABO-secretor locus (FUT2): absence in Chinese populations. J Hum Genet 44:181–184PubMedCrossRefGoogle Scholar
  30. Morgan WTJ, Watkins WM (1948) The detection of a product of the blood group O gene and the relationship of the so-called O substance to the agglutinogens A and B. Br J Exp Pathol 29:159–173PubMedGoogle Scholar
  31. Oriol R, Danilovs J, Hawkins BR (1981a) A new genetic model proposing that the Se gene is a structural gene closely linked to the H gene. Am J Hum Genet 33:421–431PubMedGoogle Scholar
  32. Oriol R, Le Pendu J, Sparkes RS, Sparkes MC, Crist M, Gale RP (1981b) Insights into the expression of ABH and Lewis antigens through human bone marrow transplantation. Am J Hum Genet 33:551–560PubMedGoogle Scholar
  33. Oriol R, Mollicone R, Couillin P, Dalix AM, Candelier JJ (1992) Genetic regulation of the expression of ABH and Lewis antigens in tissues. APMIS Suppl 27, 100:28–38Google Scholar
  34. Oriol R, Mollicone R, Cailleau A, Balanzino L, Breton C (1999) Divergent evolution of fucosyltransferase genes from vertebrates, invertebrates, and bacteria. Glycobiology 9:323–334PubMedCrossRefGoogle Scholar
  35. Oulmouden A, Wierinckx A, Petit J, Costache M, Palcic MM, Mollicone R, Oriol R, Julien R (1997) Molecular cloning and expression of a bovine α(1,3)fucosyltransferase gene homologous to the ancestor of the human FUT3-FUT5-FUT6 cluster. J Biol Chem 272:8764–8773PubMedCrossRefGoogle Scholar
  36. Palcic MM, Heerze LD, Pierce M, Hindsgaul O (1988) The use of hydrophobic synthetic glycosides as acceptors in glycosyltransferase assays. Glycoconj J 5:49–63CrossRefGoogle Scholar
  37. Peng CT, Tsai CH, Lin TP, Peng LI, Kao MC, Yang TY, Wang NM, Liu TC, Lin SF, Chang FG (1999) Molecular characterization of secretor type α(1,2)-fucosyltransferase gene deficiency in the Philippine population. Ann Hematol 78:463–467PubMedCrossRefGoogle Scholar
  38. Perrin RM, DeRocher AE, BarPeled M, Zeng WQ, Norambuena L, Orellana A, Raikhel NV, Keegstra K (1999) Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis. Science 284:1976–1979PubMedCrossRefGoogle Scholar
  39. Rajan VP, Larsen RD, Ajmera S, Ernst LK, Lowe JB (1989) A cloned human DNA restriction fragment determines expression of a GDP-L-fucose: β-D-galactoside 2-α-L-fucosyltransferase in transfected cells. Evidence for isolation and transfer of the human H blood group locus. J Biol Chem 264:11158–11167PubMedGoogle Scholar
  40. Reguigne-Arnould I, Couillin P, Mollicone R, Faure S, Fletcher A, Kelly RJ, Lowe JB, Oriol R (1995) Relative positions of two clusters of human α-L-fucosyltransferases in 19q (FUT1-FUT2) and 19p (FUT6-FUT3-FUT5) within the microsatellite genetic map of chromosome 19. Cytogenet Cell Gen 71:158–162CrossRefGoogle Scholar
  41. Rouquier S, Lowe JB, Kelly RJ, Fertitta AL, Lennon GG, Giorgi D (1995) Molecular cloning of a human genomic region containing the H blood group α(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. J Biol Chem 270:4632–4639PubMedCrossRefGoogle Scholar
  42. Sarnesto A, Köhlin T, Thurin J, Blaszczyk-Thurin M (1990) Purification of gene-encoded β-galactoside α1-2-fucosyltransferase from human serum. J Biol Chem 267:15067–15075Google Scholar
  43. Sarnesto A, Kölin T, Hindsgaul O, Thurin J, Blaszczyk-Thurin M (1992) Purification of the secretor-type β-galactoside α1-2 fucosyltransferase from human serum. J Biol Chem 267:2734–2744Google Scholar
  44. Schiff F, Sasaki H (1932) Der ausscheidungstypus, ein auf serologischem wege nachweisbares mendelndes merkmal. Klin Wochenschr 11:1426–1429CrossRefGoogle Scholar
  45. Wagner FF, Flegel WA (1997) Polymorphism of the h allele and the population frequency of sporadic nonfunctional alleles. Transfusion 37:284–290PubMedCrossRefGoogle Scholar
  46. Wang BJ, Koda Y, Soejima M, Kimura H (1997) Two missense mutations of H type α(1,2)fucosyltransferase gene (FUT1) responsible for para-Bombay phenotype. Vox Sang 72:31–35PubMedCrossRefGoogle Scholar
  47. Watkins WM (1980) Biochemistry and genetics of the ABO, Lewis and P blood group systems. Adv Hum Genet 10:1–136PubMedGoogle Scholar
  48. Watkins WM, Morgan WTJ (1955) Some observations on the O and H characters of human blood and secretions. Vox Sang 5:1–14Google Scholar
  49. Weston BW, Nair RP, Larsen RD, Lowe JB (1992) Isolation of a novel human α(1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group α(1,3/1,4)fucosyltransferase gene. J Biol Chem 267:4152–4160PubMedGoogle Scholar
  50. Yu LC, Yang YH, Broadberry RE, Chen YH, Chan YS, Lin M (1995) Correlation of a missense mutation in the human Secretor α1,2-fucosyltransferase gene with the Lewis(a+b+) phenotype: a potential molecular basis for the weak secretor allele (Se-W). Biochem J 312:329–332PubMedGoogle Scholar
  51. Yu LC, Broadberry RE, Yang YH, Chen YH, Lin M (1996) Heterogeneity of the human secretor α(1,2)fucosyltransferase gene among Lewis(a+b-) nonsecretors. Biochem Biophys Res Com 222:390–394PubMedCrossRefGoogle Scholar
  52. Yu LC, Yang YH, Broadberry RE, Chen YH, Lin M (1997) Heterogeneity of the human H blood group α(1,2)fucosyltransferase gene among para-Bombay individuals. Vox Sang 72:36–40PubMedCrossRefGoogle Scholar
  53. Yu LC, Lee HL, Chu CC, Broadberry RE, Lin M (1999) A newly identified nonsecretor allele of the human histoblood group α(1,2)fucosyltransferase gene (FUT2). Vox Sang 76:115–119PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Rafael Oriol
    • 1
  • Rosella Mollicone
    • 1
  1. 1.INSERM U504VillejuifFrance

Personalised recommendations