Abstract
α4-N-Acetylglucosaminyltransferase (α4GnT) is a glycosyltransferase that mediates transfer of GlcNAc with α1,4-linkage from UDP-GlcNAc to βGal residues preferentially present in O-glycans, forming GlcNAcα1-4Galβ-R (Nakayama et al. 1999). In the human, glycoproteins having GlcNAcα1-4Galβ-R at nonreducing terminals are exclusively limited to the mucins secreted from gland mucous cells (cardiac gland cell, mucous neck cell, pyloric gland cell) of the stomach, Brunner’s glands of the duodenum, accessory glands of the pancreaticobiliary tract, and pancreatic ducts showing gastric metaplasia (Nakamura et al. 1998) (Fig. 1). Thus, these mucous glycoproteins as a whole have been regarded as gastric gland mucous cell-type mucin, and α4GnT plays a key role in synthesizing this particular mucin which has GlcNAcα1-4Galβ-R structures. In the gastric mucin, the oligosaccharides having GlcNacα1-4Galβ-R structures were found to be attached to not only MUC6 but also MUC5AC (Zhang et al. 2001).
Expression of the gastric gland mucous cell-type mucin carrying GlcNAcα1-4Galβ-R in the human gastric mucosa, duodenal mucosa, and pancreatic duct. Mucous neck cells (a) and pyloric gland cells (b) of the gastric mucosa, Brunner’s gland cells of the duodenum (c), and pancreatic duct showing gastric metaplasia (d) produce gastric gland mucous cell-type mucin carrying GlcNAcα1-4Galβ-R. Immunostaining with HIK1083 antibody specific for G1cNAcα1-4Galβ-R; bar = 50 μm
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Nakayama, J. (2002). α4-N-Acetylglucosaminyltransferase. In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_20
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DOI: https://doi.org/10.1007/978-4-431-67877-9_20
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