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β4-Galactosyltransferase-I

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Handbook of Glycosyltransferases and Related Genes

Abstract

The enzyme β4-galactosyltransferase-I (β4GalT-I; UDP-Gal:GlcNAc β4-galactosyltransferase; EC 2.4.1.38) is a constitutively expressed, trans-Golgi resident, type II membrane-bound glycoprotein that is widely distributed in vertebrates. The protein domain structure established for β4GalT-I consists of: (1) a short NH2-terminal cytoplasmic domain of 11 or 24 amino acids depending on the protein isoform (Shaper et al. 1988; Russo et al. 1990); (2) a large COOH-terminal luminal domain containing the catalytic center (~270 amino acids) linked to a single transmembrane domain (19 amino acids) through a glycosylated peptide segment (~86 amino acids) termed the stem region. In essentially all vertebrate tissues, the primary function of β4GalT-I is to catalyze the transfer of Gal from UDP-Gal to GlcNAcβ-R, forming the N-acetyllactosamine (Galβ1-4GlcNAcβ1-R) or poly-N-acetyllactosamine structures assembled on glycoconjugates.

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Author information

Authors and Affiliations

  1. Cell Structure and Function Laboratory, Johns Hopkins Oncology Center CRB-345, The Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD, 21231-1000, USA

    Nancy L. Shaper & Joel H. Shaper

  2. Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, 21231-1000, USA

    Joel H. Shaper

Authors
  1. Nancy L. Shaper
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  2. Joel H. Shaper
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) & Koichi Honke M.D., Ph.D. (Associate Professor) (Professor) &  (Associate Professor)

  2. Glycobiology Program, Cancer Research Institute, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Minoru Fukuda Ph.D. (Professor) (Professor)

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© 2002 Springer Japan

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Shaper, N.L., Shaper, J.H. (2002). β4-Galactosyltransferase-I. In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_2

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  • DOI: https://doi.org/10.1007/978-4-431-67877-9_2

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-67996-7

  • Online ISBN: 978-4-431-67877-9

  • eBook Packages: Springer Book Archive

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