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β6-N-Acetylglucosaminyltransferase (IGnT)

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Book cover Handbook of Glycosyltransferases and Related Genes

Abstract

I-branching β6-N-acetylglucosaminyltransferase (IGnT) is a glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to β1,4-linked Gal residue in a linear poly-N-acetyllactosamine, ±Galβ1-4GlcNAcβ1-3Galβ1-4Glc(NAc)-R, forming ±Galβ1-4GlcNAcβ1-3(GlcNAcβ1-6)Galβ1-4Glc(NAc)-R. The formation of the I branch is usually followed by galactosylation with β4-galactosyltransferase I, resulting in the I antigen. The I-antigen can be further modified to express functional oligosaccharides, such as sialyl Lewis X (Fig. 1).

Biosynthetic steps of I-branches containing sialyl Lewis X termini. A linear poly-N-acetyllactosamine (i-antigen) is converted to a branched poly-N-acetyllactosamine (I-antigen) by the actions of cIGnT and β4-galactosyltransferase. By dIGnT, a branch is added to GlcNAcB1-3Galβ1-4GlcN(Ac)βl-R acceptor. I-branched poly-N-acetyllactosamines can be further modified to express sialyl Lewis X by the actions of α3-sialyltransferase and α3-fucosyltransferase (Fuc-TVII)

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Authors and Affiliations

  1. Glycobiology Program, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Minoru Fukuda

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  1. Minoru Fukuda
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Editors and Affiliations

  1. Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) & Koichi Honke M.D., Ph.D. (Associate Professor) (Professor) &  (Associate Professor)

  2. Glycobiology Program, Cancer Research Institute, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Minoru Fukuda Ph.D. (Professor) (Professor)

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© 2002 Springer Japan

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Fukuda, M. (2002). β6-N-Acetylglucosaminyltransferase (IGnT). In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_17

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  • DOI: https://doi.org/10.1007/978-4-431-67877-9_17

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-67996-7

  • Online ISBN: 978-4-431-67877-9

  • eBook Packages: Springer Book Archive

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