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N-Acetylglucosaminyltransferase-II

  • Chapter
Handbook of Glycosyltransferases and Related Genes

Abstract

The synthesis of complex N-glycans can be divided into three distinct stages. The first stage occurs primarily in the cytoplasm and rough endoplasmic reticulum, and involves the synthesis of Glc3Man9GlcNAc2-pyrophosphate-dolichol. The second stage begins with the transfer of GlcP3Man9GlcNAc2 from Glc3Man9GlcNAc2-pyrophosphate- dolichol to an Asn residue of the nascent glycoprotein followed by processing to Man5GlcNAc2-Asn-X. The third stage occurs primarily in the Golgi apparatus and starts with the action of GnT-I on Man5GlcNAc2-Asn-X followed by the removal of two mannose residues by mannosidase II to form the substrate for GnT-II (Fig. 1). GnT-II transfers GlcNAc from UDP-α-GlcNAc in the β1,2-linkage to the Manα1-6 arm of the N-glycan core, and is essential for normal complex N-glycan formation (Fig. 1).

Reaction catalyzed by GnT-II (UDP-GlcNAc:Manα1-6R [GlcNAc to Manα1-6] β-1,2-N-acetylglucosaminyltransferase-II). R = 1-4GlcNAcβ1-4GlcNAc-Asn-X

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Authors and Affiliations

  1. Program in Structural Biology and Biochemistry, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada

    Harry Schachter

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  1. Harry Schachter
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Editors and Affiliations

  1. Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) & Koichi Honke M.D., Ph.D. (Associate Professor) (Professor) &  (Associate Professor)

  2. Glycobiology Program, Cancer Research Institute, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Minoru Fukuda Ph.D. (Professor) (Professor)

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Schachter, H. (2002). N-Acetylglucosaminyltransferase-II. In: Taniguchi, N., et al. Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67877-9_10

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  • DOI: https://doi.org/10.1007/978-4-431-67877-9_10

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