• Harry Schachter


The synthesis of complex N-glycans can be divided into three distinct stages. The first stage occurs primarily in the cytoplasm and rough endoplasmic reticulum, and involves the synthesis of Glc3Man9GlcNAc2-pyrophosphate-dolichol. The second stage begins with the transfer of GlcP3Man9GlcNAc2 from Glc3Man9GlcNAc2-pyrophosphate- dolichol to an Asn residue of the nascent glycoprotein followed by processing to Man5GlcNAc2-Asn-X. The third stage occurs primarily in the Golgi apparatus and starts with the action of GnT-I on Man5GlcNAc2-Asn-X followed by the removal of two mannose residues by mannosidase II to form the substrate for GnT-II (Fig. 1). GnT-II transfers GlcNAc from UDP-α-GlcNAc in the β1,2-linkage to the Manα1-6 arm of the N-glycan core, and is essential for normal complex N-glycan formation (Fig. 1).
Fig. 1

Reaction catalyzed by GnT-II (UDP-GlcNAc:Manα1-6R [GlcNAc to Manα1-6] β-1,2-N-acetylglucosaminyltransferase-II). R = 1-4GlcNAcβ1-4GlcNAc-Asn-X


Caenorhabditis Elegans Chinese Hamster Ovary Cell Autosomal Recessive Disease Bile Duct Carcinoma Mannose Residue 
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Copyright information

© Springer Japan 2002

Authors and Affiliations

  • Harry Schachter
    • 1
  1. 1.Program in Structural Biology and BiochemistryHospital for Sick ChildrenTorontoCanada

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