Insecta (Hymenoptera)

  • Jun Mitsuhashi
Part of the Springer Lab Manual book series (SLM)


Many attempts have been made to culture bee cells without success. However, several cell lines have been established from the sawfly (Sohi and Ennis, 1981) and from endoparasitoid wasps (Lynn and Hung, 1986, 1991). The culture media used for hymenopteran cell cultures are modified Grace’s medium and modified Goodwin’s IPL-52B (see Appendix 1).


Cell Sheet Host Insect Continuous Cell Line Small Petri Dish Honeybee Apis Mellifera 
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  1. Giauffret, A., Quiot, J.M., Vago, C. and Poutiers, F. (1968) Cultures cellulaires d‘Apis mellifera. In: Barigozzi, C. (ed) Proceeding of the Second International Colloq uium on Invertebrate Tissue Culture, Villa Carlotta, 1967. Istituto Lombardo di Scienze e Lettre, Milano, pp 74–80.Google Scholar
  2. Lynn, D.E. and Hung, A.C.F (1986) Development of a continuous cell line from the insect egg parasitoid, Trichogramma pretiosum (Hymenoptera: Trichogrammatidae). In Vitro Cell. Dev. Biol. 22, 440–442.CrossRefGoogle Scholar
  3. Lynn, D.E. and Hung, A.C.F. (1991) Development of continuous cell lines from the egg parasitoids Trichogramma confusum and T. exiguum. Arch. Insect Biochem. Physiol. 18, 99–104.PubMedCrossRefGoogle Scholar
  4. Sohi, S.S. and Ennis, T.J (1981) Chromosomal characterization of cell lines of Neodiprion lecontei (Hymenoptera: Diprionidae). Proc. Entomol. Soc. Ontario 112, 45–48.Google Scholar

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© Springer Japan 2002

Authors and Affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

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